anti-Rad18(human) antibody rabbit polyclonal IgG

Code:70-023

The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (495 aa 56 kDa) as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (Ref 1~3).

Applications: (see Ref 1~3)
1) Western blotting (1000 fold dilution) 2) Indirect immunofluorescence staining
3) Immunoprecipitation
Immunogengen: GST-fusion protein containing 113 carboxyl terminal of human Rad18
Reactivity: Human Rad18. Not reactive to mouse Rad18.
Antibody: Polyclonal rabbit IgG fraction pre-adsorbed with GST affinity column.
2 mg/ml in PBS 50% glycerol 0.09% sodium azide
Storage: -20℃
Reference: This product has been used in the following references
1. Miyase S. et al. J. Biol. Chem. 280 515-524 (2005)
2. Tateishi S. et al. PNAS 97 7927-7932 (2000)
3. Watanabe K. et al. EMBO J. 23 3886-3896 (2004)

anti-XPA(human)antibody monoclonal

code:70-031

XP (Xeroderma pigmentosum) is an autosomal recessive human disease characterized by hypersensitivity to sunlight and a high incidence of skin cancer on sun-exposed skin (1). Cells from XP patients are hypersensitive to killing by UV irradiation because of a defect in nucleotide excision repair (NER). XP is classified into seven complementation groups (A~G) and a variant form (1). XPA shows the most severe symptoms. Products encoded by the XP genes function in repairing UV-induced cyclobutane pyrimidine dimmer and (6-4) photoproducts as well as chemically induced variety of DNA lesions (1).
XPA protein consists of 273 amino acids and forms a complex with many proteins such as RPA ERCC1 TFIIH、XAB1 and XAB2 which plays a role in early step of NER. The hybridoma 5F12 was constructed by Prof. K. Tanaka’s group who first cloned the XPA gene (2 3).

anti-Rad60 (S. pombe) antibody rabbit polyclonal affinity-pure

Code:63-009

Rad60 protein (406 aa 46 kDa) of S. pome is required for repair of DNA double strand breaks which occur during replication or induced by UV or gamma radiation via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.

Applications
Western blotting (1/1000~2000dilution)
Not tested for other applications
Immunogen: Recombinant full-size Rad60 protein of S. pombe produced in E. coli
Form: Whole rabbit antiserum added with 0.09% sodium azide.
Storage: Sent at 4℃ and store at -20℃

anti-Cnd2(S. pombe)antibody、rabbit antibody

Code:63-105

Cnd2 protein is a regulatory subunit of the condensin complex which is required for conversion of interphase chromatin into mitotic condensed chromosomes in S. pombe. The condensin complex also plays a role during interphase in processes of DNA repair and recombination.

Applications
1.Western blotting (500 fold dilution) (Ref.123)
2.Immunofluorescent staining (Ref. 3)
Not tested for other applications
Immunogen: Recombinant GST-Cnd2 fusion protein expressed in E. coli
specificity: Reacts with S. pombe Cnd2 protein. Not tested for
other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: shipped at -20℃ and stored at -20℃
References: This antibody was used in 3 references by Yanagida M group.

Anti-DNA polymerase β antibody (rabbit antiserum)

70-041

Reactivity: human rat mouse hamster

Immunogen: Recombinant rat DNA polymerase beta functional

Applications
1) Western blotting (1/ 1000~ 1/3000)
2) Immunoprecipitation (1/100)
3) Immunofluorescent staining (1/1000)
4) ELISA (assay dependent)

Form: 1 mg/ml in PBS 50% glycerol filter-sterilized. Azide and carrier free

Anti-DNA polymeraseδ subunit 3/p66(PolD、human) antibody monoclonal (2A1C11)

Code:70-056

DNA polymerase delta is one of the three eukaryotic DNA polymerases which are essential for chromosome replication and is also involved in nucleotide excision repair base excision repair and VDJ recombination (1 2). Subunit 3/p66 is a functionally important subunit of human polymerase delta which stabilizes polymerase delta complex and increases the affinity of polymerase delta for PCNA (3).
This product is the IgG fraction purified from serum-free culture medium of mouse hybridoma (2A1C11) by propriety chromatography under mild conditions.

Anti-Rad52　(Human) antibody affinity-purified

Catalogue number: 70-015

Human Rad52 protein is a functional and structural homolog of yeast Rad52 protein which plays a major role in genetic recombination and recombination repair by mediating strand annealing between homologous DNA strands (1). Rad51 functionally and physically interacts with Rad51 in recombination processes. It also interacts with oncogenes and tumor suppressors for the maintenance of genome stability (1).
The product was prepared by immunizing rabbit with full-size recombinant Rad52 protein expressed in E. coli and affinity-purified with the recombinant Rad52.
Using this anti-serum Rad52 protein (46 kD) was detected by Western blotting (Fig 1) at 46 kD position .This antibody was successfully used for immunoprecipitatio assay (Fig. 2) .
Usage
1) Western blotting (x 2000)
2) Immuno-precipitation
3) Detection of foci formation by indirect immunofluorescence
Specification
Reacts with: Human and mammalian Rad52 protein
Form; 1 mg/ml PBS ( 20mM Na-phosphate pH7.5 0.15M NaCl) 50% glycerol
Storage: -20 ℃
Reference
1.Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press

Anti-U1 snRNP C (U1C) antibody rat monoclonal (4H12)

Code:70-400

The spliceosomal U1C protein is critical to the initiation and regulation of precursor messenger RNA (pre-mRNA) splicing as part of the U1 small nuclear ribonucleoprotein particle (snRNP). U1C is needed for efficient complex formation of U1 snRNP with a 5’ splice site.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Applications:
1. Western blotting 2. Immunocytochemistry 3. Immunohistochemistry (frozen section)
4. ELISA Other applications have not been tested.

Anti-Rpn9 (S.cerevisiae) antibody purified

Code:62-207

The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn9 is one of the non-ATPase subunits of lid. Rpn9 plays a key role in facilitating the assembly of the 26S proteasome or in stabilizing the structure of the 26S proteasome. Rpn9 null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects.

Anti-Rpn12 (S.cerevisiae) antibody affinity purified

Code:62-209

The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit Rpt1 of the base. Rpn12 Rpt1 double mutant becomes lethal suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells the function of the 26S proteasome is completely eliminated.