E.coli RecA Protein functional

01-001

Storage： Shipped at 4°C or -20℃ and store at -20°C (-80°C for long-term storage).

Applications
1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (1).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

Form: 1.0 mg/ml in 20 mM Tris-HCl (pH 8.0) 1 mM EDTA 150 mM KCl1 mM DTT 50% glycerol

Purity: Over 90% by SDS-PAGE (CBB staining)

Background: E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity DNA annealing activity formation of D-loop and Holliday structure in homologous recombination reaction and coprotease activities that promote self-cleavages of LexA repressor lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein (2).

E.coli RecQ DNA helicase functional

01-003 01-004

Storage: Ship with ice-pack or at -20℃ and store at -20℃ or -80℃ (long period storage)

Product:Highly purified recombinant protein (>95%)

Form: 0.2～0.5 mg/ml in 20 mM Tris-HCl (pH 7.5) 1 mM EDTA 50 mM KCl 1 mM DTT 50% glycerol


Backgroud: RecQ DNA helicase of E. coli is the prototype of human
RecQ-like helicases such as Bloom and Werner hericases whose
defect causes predisposition to cancer and premature senescence.

anti-Rhp51 (S. pombe) antibody rabbit serum

63-001

Storage temmperature: Shipped at 4℃ and store at -20℃

Reactivity: Schizosaccharomyces pombe. Validated with rhp51 mutant cell for WB.

Immunogen: Purified recombinant full-length Rhp51 protein

Application
1.Western blotting (1/ 2000~1/5000 ) Fig.1
2. Immunoprecipitation (1/100-1/500)
3. Chromatin Immuno-Precipitation (Assay dependent)
4. Immunofluorescence staining (1/500 dilution). Fig. 2

Form: Rabbit antiserum added with 0.09 % sodium azide

Background: Rhp51 protein of Schizosaccharomyces pombe
(fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands.

anti-Rad22/Rad52 (S. pombe) antibody affinity-pure rabbit polyclonal

Product: Polyclonal rabbit antiserum raised against recombinant Rad22 protein (His-tag)
Applications: Western blotting (X1000-3000 tested). Immunoprecipitation (not tested)
Form: PBS 50% Glycerol
Storage: -20℃ (long period -80℃)

E.coli RuvA Protein functional

01-007 01-008

Product: Recombinant RuvA protein with no tag attached
Function:Holliday junction-specific binding protein. Forms a complex
with RuvB motor protein and together promotes branch
migration of Holliday junction in late stage of homologous
recombination.
Purity: RuvA protein over 90% by SDS-PAGE (CBB staining)
Concentration: 2.7 mg/ml (determined by BCA method)
Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol
Storage: -20C (long period -80C)

Rad51 Protein (Human) functional

Code:10-001、10-002

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs Dmc1 Rad51B Rad51C Rad51D Xrcc2 and Xrcc3 and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1 BRCA2 and P53 for the maintenance of genome stability (1).
Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still contains Gly-Ser-His at the N-terminal) it has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.

Applications
1) Studies on homologous recombination in mammals including human
2) Studies on the interaction of Rad51 protein with various proteins
3) To be used as a standard for Western blotting

E.coli RuvB Protein functional

01-009 01-010

Product: Recombinant RuvB protein with no tag attached
Function: A motor protein for Holliday junction branch migration.
Forms a complex with RuvA Holliday-junction binding
protein and together promotes branch migration of
Holliday junction in late stage of homologous
recombination.
Purity: RuvB protein over 90% by SDS-PAGE (CBB staining)
Concentration: 1.0 mg/ml (determined by BCA method)
Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol
Storage: -20C (long period -80C)

E.coli RuvC Protein functional

01-011 01-012

Backgounds:RuvC protein of E. coli resolves the recombination intermediate Holliday junction in late stage of homologous recombination and recombination repair

Product: Recombinant RuvC protein with no tag attached

Function: Holliday junction (homologous DNA duplexes held by single strands cross-over) specific endonuclease.

Purity: RuvC protein over 90% by SDS-PAGE (CBB staining)

Concentration: 2.0 mg/ml (determined by BCA method)

Form: 50% glycerol 10 mM Tris-HCl (pH7.5) 2 mM EDTA 100 mM NaCl
5 mM mercaptoethanol

anti-Rad51(Human) antibody(chicken) IgY fraction

70-005

Storage: Ship at 4℃ and store at -20℃

Reactivity: human rodents birds frog
Immunogen: Recombinant full-size human Rad51

Applications.
1.WB 2. IP 3.Chip 4.IF 5.ELISA

Purity: IgY

Form: 1 mg/ml in PBS 50% glycerol

Background: Human Rad51 protein is a functional and structural homolog of
E. coli RecA protein which plays a major role in genetic recombination
and recombination repair by mediating strand exchange reaction between
homologous DNA strands

anti-Rad51(Human) antibody (chicken) IgY-agarose conjugate

Code:70-007、70-008

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs Dmc1 Rad51B Rad51C Rad51D Xrcc2 and Xrcc3 and also with Rad52 in recombination processes. It also interacts with oncogenes and tumor suppressors such as BRACA1 BRACA2 and P53 for the maintenance of genome stability (1).
The antibody was raised by immunizing chicken with full-size recombinant Rap51 protein. The IgY fraction was purified by proprietary chromatography processes from the egg yolk. The IgY was conjugated with cyanogen bromide-activated Sepharose 4BTM *.