anti-E.coli LexA antibody 　rabbit serum

Code:61-001

E. coli LexA protein binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulon. In response to DNA damage RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result DNA repair genes and error prone polymerases are induced and DNA damage is repaired and mutation is induced (1).
The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo (2).
This product was prepared by immunizing rabbit with full-size highly-purified recombinant LexA protein. Using this antibody 23 kD LexA protein was identified in the E. coli whole-cell lysate (Fig 1) and the expression of bait constructs was identified in yeast extracts by Western blotting.

Applications
1) Studies on the SOS regulation in E .coli (3). For Western blotting; 1000~3000 fold dilution.
2) Construction and expression of a bait protein fused to LexA protein can be examined by Western blotting of the yeast extractsusing the antiserum.
Purified LexA protein is available from BioAcademia（#01-002）to be used as a positive control for Western blotting.
3) Immunohistochemistry (LexA fusion protein was detected in
transgenic Drosophila after fixation with 4% formalodehyde.)
4) Immunoprecipitation and chromatin immuno-precipitation

anti-Gal11p (S. cerevisiae) rabbit serum

code:62-001

Storage: Sent at 4℃ and store at -20℃.
Immunogen: Recombinant of S. cerevisiae Gal11 protein (1-300aa) expressed in E. coli.
Form: Whole antiserum added with 0.09% sodium azide

Applications:: Western blotting (1/500). Not tested with other applications

Background:Mediator is a protein complex which performs a very important role both in the transcription at the basal level which does not rely on transcription control factor and in the transcription activating reaction that relies on the transcription control factor. It has the characteristic of binding to the 7 amino acid repeated structure (CTD) that exists in the C terminal of the biggest subunit of RNA polymerase II. 30 kinds of subunits have already being identified as its composing substances but it is said that multiple kinds of complexes with various subunit compositions exist in the cells. Gal11p is one of the mediator subunits and is composed of 1081 amino acid residues (120 kDa) .

anti-Gcn5p (S. cerevisiae) rabbit serum

62-003

Storage: Sent at 4 ℃ and store at -20℃
Reactivity: S. pombe Gcn5 protein
Immunogen: Recombinant Gcn5 protein (His-tagged 1-300 amino acids)

Applications: Western blotting.　　(Not tested for other applications)
Form: Whole rabbit antiserum added with 0.1% sodium azide

Function: AGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histone acetylase complex which has Gcn5p as a catalyst subunit and has functions overlapping with the fundamental transcription factor TFIID which has Taf1p as a catalyst subunit. However SAGA and TFIID have different allotments and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds) TBP related protein group (4 kinds) TAF protein group (5 kinds that also pertain to TFIID) and other protein groups (>6 kinds). It has the molecular functions such as chemical modification of histone recruitment by direct interaction of transcription regulating factor on DNA and control of transcription starting reaction by TBP. Gcn5p that shows histoneacetylase activity is one of the proteins belonging to the above-mentioned Ada protein group and it is composed of 439 amino acid recidues with molecular mass of 51 kDa in the case of budding yeast.

anti-Spt3p (S. cerevisiae) rabbit serum

Code:62-005

Storage: Shipped at 4℃ and stored at -20℃

Immunogen Recombinant His-tagged S. cerevisiae Spt (1-200 aa).

Reactivity: S. cerevisiae Spt protein. Not tested with other species.

Applications:
1.Western blotting (1/2000-1/5000).
2. ELISA

Form: Whole rabbit antiserum added with 0.1% sodium azide.
Background: Spt3 is a subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia.

anti-Srb4p (S. cerevisiae) rabbit serum

62-007

Storage: Ship at 4℃ and store at -20℃.

Reactivity: S. cerevisiae Srb4 protein.

Immunogen: Recombinant full-size yeast His-tagged Srb4 protein (1-687 aa)

Applications
1. Western blotting (1/1000~1/5000 dilution)
2. Immunoprecipitation (assay dependent)
3. Chromatin Immunoprecipitation (assay dependent)

Form: Whole antiserum added with 0.1% sodium azide.

Background: Srb4 protein is subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination

anti-Sua7p/TFⅡB (S. cerevisiae) rabbit serum

62-009

Storage temperature: Ship at 4℃and store at -20℃

Reactivity: S. cerevisiae Sua7 / TFIIB protein

Immunogen: Rcombinant His-tagged full-size Sua7 protein

Applications
1.Western blotting. (1/1000~1/5000)
2.Immunoprecipitation
3.Chromatin Immuno-Precipitation
4.ELISA

Form: 0.1% sodium azide added to the antiserum

Background: The fundamental transcription factor TFIIB has the characteristics of stabilizing the DNA binding of TATA box-binding protein (TBP) and binding directly to DNA by its conformational change. Also its N terminal region binds to the RNA channel of RNA polymerase undertaking a very important role in the determination of transcription initiation point and promoter clearance. Sua7p is the TFIIB of budding yeast and is composed of 346 amino acid residues (aa)

anti-Taf4p (S. cerevisiae) rabbit serum

62-011

Storage temperature: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Taf4 protein.

Immunogen: Recombinant His-tagged N-terminal domain of Taf4p protein (1-200aa)

Applications
1. Western blotting. (1/1000~1/5000 dilution)

Form: Whole antiserum added with 0.1% sodium azide added to the antiserum

Background: The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf4p is one of the subunits of TFIID and in the case of budding yeast it is composed of 388 amino acid residues (aa). This protein contains histone folds in its interior and forms TAF octamer with Taf6p Taf9p and Taf12p.

anti-Taf6p (S. cerevisiae) rabbit serum

62-013

Storage: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Taf6 protein.

Immunogen: Recombinant His tagged Taf6 protein (1-200 aa)

Applications: Western blotting (1/1000)

Form: Whole antiserum added with 0.1% sodium azide

Background: The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf6p is one of the subunits of TFIID and in the case of budding yeast it is composed of 516 amino acid residues (aa). Taf6p is also a subunit of histoneacetylase complex SAGA which is said to have an overlapping function with TFIID. The protein contains histone folds in its interior and forms TAF octamer together with Taf4p Taf9p and Taf12p.

anti-Taf10p (S. cerevisiae) rabbit serum

Code:62-015

The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf10p is one of the subunits of TFIID and in the case of budding yeast it is composed of 206 amino acid residues (23 kDa) . Taf10p is also a subunit of histone acetylase complex SAGA which is said to have an overlapping function with TFIID. This protein contains histone folds in its interior and forms dimers with Taf3p and Taf8p each.

anti-Taf11p (S. cerevisiae) rabbit serum

Code:62-017

The basal transcription factor TFIID plays a central role in the regulation of gene expression in Eukaryota and is a large protein complex composed of TATA box-binding protein (TBP) and 14 kinds of TBP-associated factors (TAF). TFIID directly recognizes and binds to different kinds of core promoter elements that localize near the transcription initiation site and forms a scaffold for the other basal transcription factors to assemble. At the same time it transmits transcriptional activation signal originating from transcription regulating factors to RNA polymerase II. Taf11 is one of the subunits of TFIID and in the case of budding yeast it is composed of 346 amino acid residues with molecular mass of 40624.