anti-RanBPM/BP9 (human) antibody rabbit serum

71-001

Storage : Shipped at 4℃ and store at -20℃

Validation: Indpendently validated in Ref.3 for IF.

Reactivity: human monkey rodents dog

Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)
4. Immunohistochemistry (1/200 dilution. Perform heat mediated
antigen retrieval with citrate buffer (pH 6) before formalin
treated paraffin embedded sectioning)

Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex. The protein consists of 729 amino acids and is identified by Western blotting as an apparent molecular mass of 95 kD (see below). It is involved in nucleation of microtubules and controls cell growth by interacting with many protein factors.

anti-RanBP2(human)antibody　rabbit serum

Code:71-003
Storage : Shipped at 4℃ and store at -20℃
Reactivity: human monkey rodents dog
Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)
4. Immunohistochemistry (1/200 dilution. Perform heat mediated antigen retrieval with citrate buffer (pH 6) before formalin treated paraffin embedded sectioning)
Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex.

anti-p53 phospho-S46 antibody monoclonal(#36)

Code:71-115

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46 involvement of DYRK2 and ATM have been implicated.

anti-p53 p-Ser315(human）antibody、monoclonal(#18)

Code:71-117

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole (ref 1 2 & 3). However the effect of the phosphorylation on the function of p53 is mostly unknown.
This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.

anti-p53 p-Ser20(human）antibody、monoclonal（17B6)

Code:71-113

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule (1).
p53 at Ser20 is phosphorylated by Chk1 and Chk2 kinases and this enhances tetramerization stability and activity of p53 (2 3).

anti-p53 Ac-K120(human）antibody、monoclonal(10E5)

Code:71-131

Storage temperature: Ship at 4℃ and store at -20℃.

Immunogen: synthetic peptide containing acetyl-Lys315 of human p53

Isotype: mouse IgG1

Applications
1. Western blotting (~1 ug/ml) (Fig.1)
2. Immuno-precipitation (Fig.2)
3. Immunofluorescence staining (Fig.3)
4. Flow-Cytmetry (1/100)
5. ELISA

Form: purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4) 50% glycerol

Isotype: mouse IgG1 (κ)

Background: p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60 and activates transcription of proapoptotic genes BAX and PUMA .

anti-p53 Ac-K382(human)antibodymonoclonal(2B7E4)

Code:71-133

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However with stress especially with DNA damage it is activated to promote arrest of cell cycle and repair of DNA damage or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine and acetylation of lysine at various sites in the molecule.
Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase which stabilizes p53 proten (ref 1).

anti-Mdmx/Hdmx p-Ser367(mouse/human)antibody、monoclonal(#15)

Code:71-141

MdmX (synonyms: Mdm4 HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80-kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage and as a result it binds to14-3-3 and is transported into nucleus where it is　degraded by Mdm2. This process activates the p53 functions (1 2 and 3).

anti-E2F1 p-Ser364 (human) antibody monoclonal(#2)

Code:71-151

E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor (1). E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage stabilized mobilized to nucleus and activated as a transcription factor (2). It induces apoptosis by activating transcription of the p53 homolog p73 (3). E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.

anti-c-Myc p-Ser62(human)antibody、monoclonal(33A12E10)

code:71-161
Storage temperature: Shipped at 4℃ or -20℃ and stored at -20℃.
Avoid freezing by storing below -25℃

Validation and caution: Specificity of this antibody have been independently validated and caution of the use described in Ref.3
which is recommended to be read by the users..
Reactivity: Human and mouse.
Immunogen: Synthetic peptide containing phospho-Ser62 of cMyc

Applications
1. Western blotting (~1ug/ml Fig.1)
2. Immunofluorescence staining (0.5~1 μg/ml Fig.2)
3. Immunohistochemistry (5 μg/ml Perform heat mediated antigen
retrieval with citrate buffer pH 6 before formalin treated
paraffin embedded sectioning)
4. Flow cytometry (Use 1 μg for 106 cells)
5. Indirect ELISA (Assay dependent concentration)　　

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol. Azide- and carrier-free.
Isotype: Mouse IgG2b (κ)