抗ジフテリア毒素抗体、ウサギPC抗血清

Diphtheria toxin (535 aa 58 kDa) is an exotoxin secreted as a proenzyme by Corynebacterium diphtheriae the pathogen bacterium that causes diphtheria. The toxin gene is encoded by a bacteriophage. The proemzyme undergoes proteolytic nicking and reduction of disulfide bond giving rise to subunit A (21 kDa) and B (37 kDa). Subunit B (C-terminal) recognizes cellular receptor HB-EGF receptor and subunit A (N-terminal) catalyzes the ADP-ribosylation of eukaryotic elongation factor-2 (eEF2) inactivating this protein.
Applications:
1. Western blotting (1/2000 dilution).
2. ELISA (1/2000~1/7000 dilution. Could detect 1 ng or less of the toxin)
3. Immunoprecipitation (1/200~ 1/1000 dilution depending on assay)
4. Dot blotting (1/1000 dilution. Could detect 1 ng or less of the toxin) 　
Immunogen: Purified nontoxic mutant Diphtheria toxin CRM 197 (Ref 1 Product #01-515)

ジフテリア毒素変異体　CRM197 active (non-toxic)

製品番号:01-515
製品：Corynebacterium diphteriae CRM197変異株の培養上清より高度に精製（95%以上の純度）
性状： 1.0 mg/ml in 20 mM Tris-HCl pH 7.2 0.15M NaCl 10% glycerol
性質：CRM197 (Cross-Reacting Material) は52番目のGlyがGluに変異し
ているために細胞毒性をもたない。
用途：毒素変異体CRM197がHB-EGFに結合すると、HB-EGFの細胞増殖作用が
抑制されるので、CRM197はHB-EGFの阻害剤として利用されている

文献：Mitamura T. et al. J. Biol. Chem. 272: 27084 (1997)
Prenzel N. et al. Nature 402: 884 (1999)