anti-FcεR1α(human IgE receptor) antibody mouse monoclonal (CRA1)

Code:72-001

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human house musk shrew

Immunogen: Recombinant extracellular portion of human
FcεR1α(corresponding to amino acids Met-26-197 where signal peptide
is 1-25)
Epitope: 26-110 amino acids

Applications:
1) Western blotting (～1ug/ml)
2) Flow Cytometry (FC) (1~5 ug/ml)
3) IHC-P IHC-F (~1 ug/ml)
4) Titration of IgE-bound receptor in combination with CRA2 antibody
5) Promotion of migration of basophils (Ref.5)

Isotype: IgG2b

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4) 50% glycerol filter-sterilized azide and carrier free

Background:FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α one β and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1αcomplex plays an important role in triggering allergic responses.

anti-FcεRIα（human IgE receptor) antibody mouse monoclonal(CRA2)

Code:72-005

FcεR1α is subunit of the high affinity receptor of IgE to which it directly
binds.It plays important role in allergic response.
Product:The CRA2 (AER24) monoclonal antibody specific to FcεR1α
State: Purified IgG1(κ)1 mg/ml in PBS (pH 7.4) 50% glycerol sterile-filtered azide-
free
Applications:1) Western blotting (~1 ug/ml) 2) FACS 3) Immunohistochemistry
4)Quantification of FcεR1 bound by IgG in combination wity CRA2
References: The product was described and used in reference 2.
1.Ra C et al Nature 341:752 (1989)
2.Takai T et al. Biosci. Biotechnol. Biochem. 64: 1856 (2000)(PubMed)
Related product: 72-001 Anti-FcεR1α human IgE receptor monoclonal (CRA1)

anti-FcεRIα（human IgE receptor) antibody (CRA2) Biotinylated

72-007

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)

Epitope: Amino acids 110-197 of FcεR1α

Applications:
1) Western blotting (～1ug/ml)
2) Flow-Cytometry
3) Immunohistochemistry (Paraffin and Frozen) and immunocytochemistry
4) Titration of IgE-bound fraction of the FcεR1α using CRA1 and CRA2 antibodies

Isotype: IgG1 (κ)

Purity: This product is the IgG fraction purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

Form: 1mg/ml in PBS (pH 7.4) 50% glycerol filter-sterilized azide and carrier free. Biotinylated


Background:FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α one β and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1αcomplex plays an important role in triggering allergic responses.
The CRA2 (AER24) monoclonal antibody reacts with the FcεR1αsubunit on a region that overlaps the region of the IgE binding site thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1α it does not compete with IgE for the receptor binding. Combining the two antibodies one can quantitatively measure the amounts of the IgE-bound FcεR1α.

anti-FcεRIα（human IgE receptor) antibody(CRA2) FITC-labeled

Code:72-008

FITC-labeled IgG
FcεR1α is subunit of the high affinity receptor of IgE to which it directly binds.It plays important role in allergic response.
Product:The CRA2 (AER24) monoclonal antibody specific to FcεR1α
State: Purified IgG1(κ)1 mg/ml in PBS (pH 7.4) 50% glycerol sterile-filtered azide-free
Applications:1) FACS 2) Immunohistochemistry
3)Quantification of FcεR1 bound by IgG in combination wity CRA2
References: The product was described and used in reference 2.
1.Ra C et al Nature 341:752 (1989)
2.Takai T et al. Biosci. Biotechnol. Biochem. 64: 1856 (2000)(PubMed)
Related product: 72-001 Anti-FcεR1α human IgE receptor monoclonal (CRA1)

anti-FcεRIα（human IgE receptor) antibody (CRA1) Biotinylated

72-003

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human house musk shrew

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)
Epitope: 26-110 amino acids

Applications:
1) Western blotting (～1ug/ml)
2) Flow Cytometry (FC) (1~5 ug/ml)
3) IHC-P IHC-F (~1 ug/ml)
4) Titration of IgE-bound receptor in combination with CRA2 antibody (Ref.3)
5) ELISA

Isotype: IgG2b

Form: Biotynylated monoclonal antibody (IgG) 1.6 mg/ml (depends on Lot) in PBS (pH 7.4) 50% glycerol filter-sterilized azide free

anti-FcεRIα（human IgE receptor) antibody(CRA1) FITC-labeled

Code: 72-004

FcεR1α is subunit of the high affinity receptor of IgE to which it directly binds. FcεR1 is a tetrameric complex consisting of one α one β and twoγ subunis. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses.
The CRA1 (AER37) monoclonal antibody reacts with the FcεR1α subunit on a region that does not overlap the region of the IgE binding site thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1α it compete with IgE for the receptor binding. Combining the two antibodies one can quantitatively measure the amounts of the IgE-bound FcεR1α.
The IgG fraction is purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. This product is an FITC-labeled IgG ([FITC]/[IgG] = 6.1) produced from the IgG fraction.
Applications
1) Western blotting (～1 ug/ml) 2) FACS 3) Immunohistochemistry
4) Titration of IgE-bound in combination with CRA2 antibody
Form: Purified monoclonal antibody (IgG) 1.7 mg/ml in PBS (pH 7.4)
50% glycerol sterile-filtered azide free
Storage: -20℃
References: This product was used in references 3 and 4.
1. Ra C et al. Nature 341:752 (1989)
2. Hakimi J et al. J. Biol. Chem 265:22079 (1990)
3. Yamagchi M J. Immuno1. 162:5545 (1999)
4. Suzukawa M et al. Int. Immunol. : 1249 (2005)

anti-CD40 antibody mouse monoclonal (5C3) biotinylated

Code:72-031

CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation differentiation immunoglobulin isotype switching germinal center formation and stimulation of the humoral memory response.
This antibody reacts with a 45-48 kDa type I integral membrane glycoprotein present on peripheral blood and tonsillar B cells but not expressed on terminally differentiated B cells.
The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and purified under mild conditions by proprietary chromatography processes.

anti-CD40 antibody mouse monoclonal (5C3) FITC-conjugated

Code:72-032

CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation differentiation immunoglobulin isotype switching germinal center formation and stimulation of the humoral memory response.
This antibody reacts with a 45-48 kDa type I integral membrane glycoprotein present on peripheral blood and tonsillar B cells but not expressed on terminally differentiated B cells.
The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and purified under mild conditions by proprietary chromatography processes.

anti-CD40 antibody mouse monoclonal (5C3)

Code:72-030

CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells playing a role in both humoral and cell-mediated immune responses.
Activation of CD40 on B cells by CD40 ligand causes B cell proliferation differentiation immunoglobulin isotype switching germinal center formation and stimulation of the humoral
memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. Within the cell the CD40 molecule acts as a transmembrane signal transducer that leads to
activation of intracellular kinases and transcription factors.

Applications:
1. Flowcytometry (Ref 2)
2.Immunohistochemistry(acetone or zinc-fixed
or frozen section; indirect immuno-staining)
3. Stimulation of proliferation of B cell and
dendritic cells. Enhancement of proinflamentory
cytokine production in human monocytes such
as TNF-a IL-6 and IL-8 (Ref 3)