Cholera toxin B subunit from Vibrio Cholerae active

01-525

Cholera toxin is a complex consisting of one molecule of A-subunit (27.2 kD) and 5 molecules of B-subunit (11.6 kD). It adsorbs to GM1 ganglioside on the surface of target cells by the B subunit

Applications
1. Adjuvant for mucosal immunity. Stimulate B-lymphocytes. Vaccine
development
2. Tract tracing in neurological research taking advantage of GM1
ganglioside binding and retrograde transport.

Form：1 mg/ml in 20 mM Tris-HCl pH 6.8、0.2M NaCl 10% glycerol Filter-sterilized

anti-FcεR1α(human IgE receptor) antibody mouse monoclonal (CRA1)

Code:72-001

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human house musk shrew

Immunogen: Recombinant extracellular portion of human
FcεR1α(corresponding to amino acids Met-26-197 where signal peptide
is 1-25)
Epitope: 26-110 amino acids

Applications:
1) Western blotting (～1ug/ml)
2) Flow Cytometry (FC) (1~5 ug/ml)
3) IHC-P IHC-F (~1 ug/ml)
4) Titration of IgE-bound receptor in combination with CRA2 antibody
5) Promotion of migration of basophils (Ref.5)

Isotype: IgG2b

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4) 50% glycerol filter-sterilized azide and carrier free

Background:FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α one β and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1αcomplex plays an important role in triggering allergic responses.

anti-FcεRIα（human IgE receptor) antibody mouse monoclonal(CRA2)

Code:72-005

FcεR1α is subunit of the high affinity receptor of IgE to which it directly
binds.It plays important role in allergic response.
Product:The CRA2 (AER24) monoclonal antibody specific to FcεR1α
State: Purified IgG1(κ)1 mg/ml in PBS (pH 7.4) 50% glycerol sterile-filtered azide-
free
Applications:1) Western blotting (~1 ug/ml) 2) FACS 3) Immunohistochemistry
4)Quantification of FcεR1 bound by IgG in combination wity CRA2
References: The product was described and used in reference 2.
1.Ra C et al Nature 341:752 (1989)
2.Takai T et al. Biosci. Biotechnol. Biochem. 64: 1856 (2000)(PubMed)
Related product: 72-001 Anti-FcεR1α human IgE receptor monoclonal (CRA1)

anti-FcεRIα（human IgE receptor) antibody (CRA2) Biotinylated

72-007

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)

Epitope: Amino acids 110-197 of FcεR1α

Applications:
1) Western blotting (～1ug/ml)
2) Flow-Cytometry
3) Immunohistochemistry (Paraffin and Frozen) and immunocytochemistry
4) Titration of IgE-bound fraction of the FcεR1α using CRA1 and CRA2 antibodies

Isotype: IgG1 (κ)

Purity: This product is the IgG fraction purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

Form: 1mg/ml in PBS (pH 7.4) 50% glycerol filter-sterilized azide and carrier free. Biotinylated


Background:FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α one β and two γ subunits. The latter two subunits are required for signal transduction activity. The FcεR1αcomplex plays an important role in triggering allergic responses.
The CRA2 (AER24) monoclonal antibody reacts with the FcεR1αsubunit on a region that overlaps the region of the IgE binding site thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1α it does not compete with IgE for the receptor binding. Combining the two antibodies one can quantitatively measure the amounts of the IgE-bound FcεR1α.

anti-FcεRIα（human IgE receptor) antibody(CRA2) FITC-labeled

Code:72-008

FITC-labeled IgG
FcεR1α is subunit of the high affinity receptor of IgE to which it directly binds.It plays important role in allergic response.
Product:The CRA2 (AER24) monoclonal antibody specific to FcεR1α
State: Purified IgG1(κ)1 mg/ml in PBS (pH 7.4) 50% glycerol sterile-filtered azide-free
Applications:1) FACS 2) Immunohistochemistry
3)Quantification of FcεR1 bound by IgG in combination wity CRA2
References: The product was described and used in reference 2.
1.Ra C et al Nature 341:752 (1989)
2.Takai T et al. Biosci. Biotechnol. Biochem. 64: 1856 (2000)(PubMed)
Related product: 72-001 Anti-FcεR1α human IgE receptor monoclonal (CRA1)

anti-FcεRIα（human IgE receptor) antibody (CRA1) Biotinylated

72-003

Storage: Ship at 4℃ and store at -20℃ (Do not store below -20℃)

Reactivity: human house musk shrew

Immunogen: Recombinant extracellular portion of human FcεR1α (corresponding to amino acids Met-26-197 where signal peptide is 1-25)
Epitope: 26-110 amino acids

Applications:
1) Western blotting (～1ug/ml)
2) Flow Cytometry (FC) (1~5 ug/ml)
3) IHC-P IHC-F (~1 ug/ml)
4) Titration of IgE-bound receptor in combination with CRA2 antibody (Ref.3)
5) ELISA

Isotype: IgG2b

Form: Biotynylated monoclonal antibody (IgG) 1.6 mg/ml (depends on Lot) in PBS (pH 7.4) 50% glycerol filter-sterilized azide free

anti-FcεRIα（human IgE receptor) antibody(CRA1) FITC-labeled

Code: 72-004

FcεR1α is subunit of the high affinity receptor of IgE to which it directly binds. FcεR1 is a tetrameric complex consisting of one α one β and twoγ subunis. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses.
The CRA1 (AER37) monoclonal antibody reacts with the FcεR1α subunit on a region that does not overlap the region of the IgE binding site thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1α it compete with IgE for the receptor binding. Combining the two antibodies one can quantitatively measure the amounts of the IgE-bound FcεR1α.
The IgG fraction is purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. This product is an FITC-labeled IgG ([FITC]/[IgG] = 6.1) produced from the IgG fraction.
Applications
1) Western blotting (～1 ug/ml) 2) FACS 3) Immunohistochemistry
4) Titration of IgE-bound in combination with CRA2 antibody
Form: Purified monoclonal antibody (IgG) 1.7 mg/ml in PBS (pH 7.4)
50% glycerol sterile-filtered azide free
Storage: -20℃
References: This product was used in references 3 and 4.
1. Ra C et al. Nature 341:752 (1989)
2. Hakimi J et al. J. Biol. Chem 265:22079 (1990)
3. Yamagchi M J. Immuno1. 162:5545 (1999)
4. Suzukawa M et al. Int. Immunol. : 1249 (2005)