anti-RB p-Ser795(human)antibody、monoclonal(28B5)

Code:71-171

Retinoblastoma protein (Rb) the tumor suppressor product of the retinoblastoma susceptibility gene is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation (1). At the transcriptional level Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1 PU1 ATF2 UBF Elf-1 and c-Abl (2-4). Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by phosphorylation which is catalyzed by the cyclin-dependent protein kinases

anti-RB p-Thr821(human)antibody、monoclonal(24A7)

Code:71-173

Retinoblastoma protein (Rb) the tumor suppressor product of the retinoblastoma susceptibility gene is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation (1). At the transcriptional level Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1 PU1 ATF2 UBF Elf-1 and c-Abl (2-4). Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by phosphorylation which is catalyzed by the cyclin-dependent protein kinases.

anti-Cnd2(S. pombe)antibody、rabbit antibody

Code:63-105

Cnd2 protein is a regulatory subunit of the condensin complex which is required for conversion of interphase chromatin into mitotic condensed chromosomes in S. pombe. The condensin complex also plays a role during interphase in processes of DNA repair and recombination.

Applications
1.Western blotting (500 fold dilution) (Ref.123)
2.Immunofluorescent staining (Ref. 3)
Not tested for other applications
Immunogen: Recombinant GST-Cnd2 fusion protein expressed in E. coli
specificity: Reacts with S. pombe Cnd2 protein. Not tested for
other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: shipped at -20℃ and stored at -20℃
References: This antibody was used in 3 references by Yanagida M group.

anti-Cut5/Rad4 (S.pombe) antibody、rabbit antibody

code:63-107

Cut5/Rad4/Dre3 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1 2). It interacts with chromatin proteins to form the complex required for the initiation and progression DNA synthesis. It contains 4 BRCT domains and the molecular mass is 74.1 kDa with 648 amino acids.
Applications
1. Western blotting (500 fold dilution) 2. Not tested for other applications
Immunogen: Recombinant GST-fused with the N-terminal half of Cut5 protein.
specificity: Reacts with S. pombe Cut5/Rad4 protein. Not tested for other species.
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: -20 ℃ (long period -70℃)
References: This antibody was used in the following references.
1. Saka Y. et al. Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2 a protein with BRCT motif with Cut5 and Chk1.Genes Dev.11:3387 (1997)
2. Saka Y. et al. Fission yeast cut5 links nuclear chromatin and M phase regulation in the replication checkpoint control. EMBO J. 13:5319 (1994)

anti-Cut15(S. pombe)antibody、rabbit antibody

Code:63-113

Cut15 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1 2). It interacts with importin alpha (Imp1p) which together functions in nucleocytoplasmic transport and cell cycle progression . It contains 4 BRCT domains and the molecular mass is 60.4 kDa with 542 amino acids.

Applications
1. Western blotting (100-1000 fold dilution)
Not tested for other applications

Immunogen: Recombinant GST-fusion protein with the full length of Cut15 protein

Specificity: Reacts with S. pombe Cut15 protein. Not tested for other species

Form: Rabbit antiserum added with 0.05 % sodium azide

Storage: Shipped at 4 ℃ or -20℃ and store at -20℃

anti-Dis2(S. pombe)antibody、rabbit antibody

Code:63-119

Schizosaccharomyces pombe Dis2 is a serine/threonine protein phosphatase which is highly similar to mammalian type 1 phosphatase (PP1). Protein phosphatases are known to play critical roles in cell cycle regulation in fission yeast. Fission yeast has two type 1 protein phosphatases Dis 2 and Sds21. They are 37 kDa proteins and their amino acid sequences are 80% identical to each other and to mammalian PP1 homologs. Dis 2 and Sds21 are necessary for mitotic chromosome disjunction and have overlapping functions. Their disruptants are lethal only when both genes are disrupted. Dis 2 is known to be enriched in nuclei.
1. Immunoblotting (dilution: 1/300~1/1000)
2. Immunoprecipitation

anti-Dis2(P-T316)(S. pombe)antibody、rabbit antibody

Code: 63-121

Phoshoprotein phosphatase DNA damage checkpoint microtubule cytoskeleton organization rRNA processing signal transduction signal transduction mitotic cell cycle homologous chromosome segregation
Backgroud: S. pombe dis2 gene encodes Serine/threonine-protein phosphatase PP1-1 (327 aa 37.6 kDa) which plays essential role in cell cycle control and required for exit from mitosis.
Dis2 protein is phosphorylated at Thr 316 in mitosis
Applications
1. Western blotting (1/1000~1/2000 dilution). Not tested for other applications
Immunogen: Synthetic peptide NWHMT(PO3)PPRKN conjugated to KLF
Specificity: Reacts with S. pombe Dis2 protein phosphorylated at Thr316. Not tested with other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: Shipped at 4℃ and upon arrival centrifuge briefly aliquot and store at -20℃ .

anti-Dis3(S. pombe)antibody、rabbit antibody

code:63-123

S. pombe Dis3 protein is an essential component for mitotic segregation (ref.1). It is a component of the exosome 3->5 exoribonuclease complex. It is required for the 3-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran and has a 3-5 exonuclease activity. It is composed of 970 amino acids with molecular mass of 110 kDa. It is highly conserved functionally and structurally from yeast to human.

anti-Pad1(S. pombe)antibody、rabbit antibody

Code:63-133

Storage: Shipped at 4℃or -20℃ and stored at -20℃
Reactivity: S. pombe
Immunogen: Recombinant S. pombe full-length Pad1

Applications:
1. Immunoblotting (dilution: 1/300~1/1000)
2. Immunoprecipitation
Form: Rabbit antiserum added with 0.05 % sodium azide

Background:Schizosaccharomyces pombe Pad1 a 35 kDa protein is a component of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Transcription factor Pap1 is controlled by the functional interaction between the positive regulator Pad1 and negative regulator Crm1. Both proteins are essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine and other drugs such as cycloheximide and caffeine.

anti-Ppa2(S. pombe)antibody、rabbit antibody

Code:63-135

Schizosaccharomyces pombe Ppa2 is a type 2A-like serine/threonine-protein phosphatase catalytic subunit whose polypeptide sequence has ~80% identity to those of mammalian type 2A phosphatases. Ppa2 determines the sensitivity to okadaic acid which is an inhibitor of protein serine/threonine phosphatases. The loss of the ppa2 gene causes cells to be hypersensitive to the okadaic acid. Ppa2 plays important roles in cell cycle control. It may be involved in controlling the entry into mitosis possibly acting as an inhibitor (ref.1). Ppa2 is abundant in the cytoplasm in contrast to the type 1-like phosphatase Dis2 which is enriched in the nucleus. Thus Ppa2 may perform major functions outside the nucleus.