anti-Rad51(S. cerevisiae) antibody rabbit serum

62-101

Storage: Sent at 4℃ and store at -20℃

Reactivity: S.cervisiae Rad 51 protein. The specificity of reaction

Immunogen: Recombinant His-tagged Sc Rad51 protein (full-size)

Applications
1) Western blotting (1/1000~1/10000 dilution) (Fig. 1 2)
2) Immunoprecipitation.
3) Chromatin Immunoprecipitaiton
4) Immunoflorescence staining

Form: Whole antiserum added with 0.09 % sodium azide

Background: S. cerevisiae Rad 51 protein　(400 aa 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl Rad55 Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes

Rad52 Protein (Human) functional

Code:10-003、10-004

Backgrounds:Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1). Rad52 functionally and physically interacts with Rad51 Dmc1 and RPA in recombination processes. In radiation damaged cell Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci

Product:Expressed as a tagged protein in E. coli and highly purified

Form: 1 mg/ml in 20 mM Tris-HCl pH8.0
　　　100 mM KCl 2 mM 2ME 0.5 mM EDTA 20% glycerol

Reference: This product was used in reference 3
1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press
2. Kitao H & Yuan Z M J. Biol. Chem. 277:48944 (2002)
3. Kurumizaka H et al. J Biol Chem 276: 35201

Related Products：
70-011 anti-Rad52 (human) rabbit affinity purified antibody
10-001 Rad51 (human) protein
70-001 Anti-Rad51 (human) rabbit antiserum

Anti-Rad52　(Human) antibody affinity-purified

Catalogue number: 70-015

Human Rad52 protein is a functional and structural homolog of yeast Rad52 protein which plays a major role in genetic recombination and recombination repair by mediating strand annealing between homologous DNA strands (1). Rad51 functionally and physically interacts with Rad51 in recombination processes. It also interacts with oncogenes and tumor suppressors for the maintenance of genome stability (1).
The product was prepared by immunizing rabbit with full-size recombinant Rad52 protein expressed in E. coli and affinity-purified with the recombinant Rad52.
Using this anti-serum Rad52 protein (46 kD) was detected by Western blotting (Fig 1) at 46 kD position .This antibody was successfully used for immunoprecipitatio assay (Fig. 2) .
Usage
1) Western blotting (x 2000)
2) Immuno-precipitation
3) Detection of foci formation by indirect immunofluorescence
Specification
Reacts with: Human and mammalian Rad52 protein
Form; 1 mg/ml PBS ( 20mM Na-phosphate pH7.5 0.15M NaCl) 50% glycerol
Storage: -20 ℃
Reference
1.Friedberg EC et al. DNA Repair and Mutagenesis 2nd ed. ASM Press

T4 SSB (gene 32) protein functional

Code:02-040、02-040-5

T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity (12). It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
Applications: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA (1)
2. Increase specificity and yields of long PCR products (3)
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
Form: 10 mg/ml in 20mM Tris-HCl (pH 8.0) 100mM NaCl 0.5mM dithiothreitol 1mM EDTA 50% glycerol
Storage: -20℃

E. coli SSB (Single-Stranded DNA Binding) protein functional

Code: 02-042、02-042-5

E.coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity (1 2). It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E.coli highly purified. The molecular mass is 18.9 kDa.
Applications:
1. Functional single-stranded DNA-binding protein for studying DNA replication and recombination
2. Enhancement of the specificity and yield of PCR
Form: 5 mg/ml in 20mM Tris-HCl (pH 7.6) 200mM NaCl 1mM dithiothreitol 1mM EDTA 50% glycerol
Storage: -20 C
Quality: Greater than 95% purity as determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.

Taq SSB (Single-Stranded DNA Binding) protein functional

Code:02-044

Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNA replication and recombination (2). Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa same as that of the natural protein.
Applications:
Stabilizes single-stranded DNA in DNA replication repair and recombination
Storage conditions:
50mM Tris-HCl (pH 8.0) 200mM NaCl 0.1mM dithiothreitol 0.5mM EDTA 50% glycerol
Store at -20℃
Activity:
Single-stranded DNA binding activity was confirmed (Fig.2).
Concentration:
1.0 mg/ml
Quality Assurance:
Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.

Taq RecA protein functional

Code:02-048

Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase DNA annealing and exchanging of strands between two recombining DNA double helices similar to E.coli RecA protein but the optimal temperature is between 65~75℃ (1). Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.

Applications:
1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA (2)
3) Visualization of DNA with electoron microscopy due to nucleofilament formation.

Form: 1 mg/ml in 50mM Tris-HCl (pH 8.0) 200mM NaCl 1mM EDTA 50% glycerol
Store at -20℃

Activity:
　The activity of single-stranded DNA-dependent ATPase was confirmed.

Quality Assurance: Single-strand dependent ATPase activity.
Greater than 90% of protein determined by SDS-PAGE (CBB staining) (Fig.1)
The absence of endonucleases and exonucleases was confirmed.

T4 DNA Ligase with reaction buffer and ATP

Code:02-050、02-050-5

Bacteriophage T4 derived DNA ligasecatalyzes the formation of phosphodiester bonds between 3’-OH termini and 5’-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA RNA or DNA/RNA hybrids。
　　T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa.　
Applications:
1) Insertion of DNA fragment into a vector
2) Linker (or Adaptor) ligation with DNA fragment
Storage conditions:
10mM Tris-HCl (pH 7.6) 50mM KCl 0.1mM EDTA 1mM dithiothreitol 50% glycerol
Store at -20℃
Concentration:
400 U/l where one unit is the amount of enzyme that ligates more than 90% of 6 g of DNA-HindIII fragments in a 20l mixture in 30 minutes at 16℃.
Quality Assurance:
Greater than 95% protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.

E. coli Ribonuclease H (RNase H) with reaction buffer

code:02-060、02-060-5

Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

Applications
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA (1 2)
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT) (3)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA (4)

Specifications
Form: 50 units/ul in 20mM Tris-HCl (pH 7.5) 100mM KCl 1mM DTT 50% Glycerol
Specific Activity: 100000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃.
Storage: at -20℃
Quality Assurance: Greater than 95% protein determined by SDS-PAGE (CBB staining) (Fig.1). Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37℃.

DNA polymerase kappa (human) functional

code:10-105

Mammalian DNA polymerase κ a member of the UmuC/DinB nucleotidyl transferase superfamily has been implicated in spontaneous mutagenesis (1). Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10-3 and 2 x 10-3 respectively. These error rates are high when compared to those of most other DNA polymerases (2). DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions (3).
This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa) and highly purified by several steps of chromatography (2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed by a user researcher even if it was diluted 8000-fold.
Application
1) Functional studies on translesion DNA synthesis by DNA polymerase kappa
2) Antigen for western blotting and ELISA by anti-Pol kapp