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Code:74-001
Glyoxalase I (GLO1) is an enzyme that plays a role in the detoxification of methylglyoxal (MG) a side-product of glycolysis via condensation with glutathione to produce S-lactoyl-glutathione. GLO1 is a zinc metalloenzyme whose crystal structure has been solved. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric and its sequence is well conserved. GLO1 is found over-expressed in some tumors. GLO1 has also been suggested to be involved in anxiety diseases autism and Alzheimer’s disease.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Applications
1. Western blotting (~X 1000) 2. Immunocytochemistry 3. ELISA
Other applications are not tested.
Immunogen: Recombinant GST-fused mouse glyoxalase I (full length)
Isotype: Rat IgG2b κ
Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized
Specificity: Specific to human simian and mouse glyoxalase I.
Other species are not tested.
References:
1. ChenF. et al. (2004) Role for glyoxalase I in Alzheimer’s disease.” Proc. Natl. Acad. Sci. USA 101: 7687–7692.
2. Junaid M.A. et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J. Med. Genet. A. 131: 11–17.
3. Hovatta I. et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice Nature 438: 662–666.
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