Anti-ATF6 alpha antibody mouse monoclonal (1-7)

73-500

Applications:
1. Western blotting
2. Immunoprecipitation (IP)
3. immunofluorescence staining.

Immunogen: Recombinant ATF6 alfa; (His-tagged amino-terminal fragment of ATF6 alfa;)


Isotype: mouse IgG2a kappa;

Form: purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: specific to human ATF6 alfa no cross reactivity with mouse ATF6 alfa;




ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress. When unfolded proteins accumulate in the ER ATF6 alpa; is cleaved by regulated intramembrane proteolysis. The resulting amino-terminal fragment translocates to the nucleus and activates transcription by binding to ER stress-response elements present in the promoter regions of ER stress-inducible genes

The monoclonal antibody was characterized in the laboratory of Professor Kazutoshi Mori of Kyoto University.

Anti-ATF6 alpha antibody mouse monoclonal (37-1)

code:73-505

ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress. When unfolded proteins accumulate in the ER ATF6 alfa is cleaved by regulated intramembrane proteolysis. The resulting amino-terminal fragment translocates to the nucleus and activates transcription by binding to ER stress-response elements present in the promoter regions of ER stress-inducible genes including those encoding ER chaperones and components of ER-associated degradation. ATF6 consists of two closely related factors ATF6 alfa and ATF6 beta in mammals. ATF6 alfa but not ATF6 beta plays a pivotal role in transcriptional control.
The monoclonal antibody was characterized in the laboratory of Professor Kazutoshi Mori of Kyoto University. The antibody was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Applications: (Detailed Protocol is given below)
1. Western blotting
2. Immunoprecipitation (IP) (less efficient than clone1-7)
This antibody does not work for immunofluorescence analyses.
Immunogen: Recombinant ATF6 (amino-terminal fragment of ATF6 fused to GST)
Isotype: mouse IgG1 Epitope: not determined
Form: purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized
Specificity: Reactive to human and mouse ATF6However clone 1-7 antibody (#73-500) is recommended for human cells.
Storage: Shipped at 4℃ or -20℃ and stored at -20℃
Data Link Swiss-Prot P18850 (human ATF6 alpha)
References: This antibody is described in Ref 1.
1.Mori K “Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!”Mol Biol Cell 21: 11435-8 (2010) PMID:20219975