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code:70-657
SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination which targets proteins for degradation SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport gene expression cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human; SUMO1 SUMO2 and SUMO3. SUMO2 and 3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO2/3 forms poly-(SUMO) chains is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Molecular mass: SUMO2; proform 10871 Da with 95 aa (94-95 aa are removed from proform). SUMO3; proform 11637 Da with 103 aa (93-103 aa are removed from proform).
Applications:
1. Western blotting (1/1000)
2. Immunocytochemistry/Immunofluorescence staining (1/100~1/500)
3. Immunohistochemistry frozen section (1/100~1/500)
4. ELISA (Assay dependent concentration)
Other applications have not been tested.
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