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Code:70-305
The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .
Applications
1. Western blotting (1/500 ~1/2000 dilution)
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry (1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells it accumulates into the nuclear pores as examined by immunofluorescence staining..
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