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Code:70-133
Storage: Sent at 4℃ and upon arrival spin-down and store at -20℃
Reactivity: human mouse rat
Immunogen: Recombinant GST-human CDC6 (1-326 amino acids) expressed in E. coli
Validation: Specificity of reaction has been validated with siRNA in WB (Ref.5 & 7)
Applications:
1. Western blotting (1/1000~3000)
2. Immunoprecipitation （1/200）
3. Chromatin Immuno-Precipitation (assay dependent)
4. Immunofluorescence staining (1/200)
Form: 1 mg/ml in PBS with 50% glycerol. Filter-sterilized. Azide and carrier free
Function: CDC6 (Cell Division Control Protein 6 homolog) (human; 560 aa 62.7 kDa) is involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular location: The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Purification: The antiserum was first adsorbed with GST conjugated agarose column and
then the antibody in the pass-through fractions were purified with GST-CDC6 conjugated column.
Data base Link: uniprot/Q99741 (CDC6_HUMAN)
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