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code:63-139
Rad21 protein (628 aa 67.8 kDa) is a cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition this protein is cleaved by Cut1 and dissociates from
chromatin allowing sister chromatids to segregate. Also involved in the DNA double-strand-break (DSB) repair system Hyperphosphorylated during S and G2 phases.
Proteolytic cleavage of a fraction of hyperphosphorylated form at the onset of anaphase may be essential for the proper progression of anaphase and sister chromatid separation. Belongs to the rad21 family conserved to mammals.
Applications
1. Western blotting (1/1000~1/4000 dilution) (Ref.1 & 2)
Not tested for other applications
Immunogen: Recombinant full-size S. pombe Rad21 protein (His-tagged) expressed in E. coli
Specificity: Reacts with S. pombe Rad21 protein. Not tested with other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: Shipped at 4℃ and stored at -20℃ or below
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