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Code:62-209
The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit Rpt1 of the base. Rpn12 Rpt1 double mutant becomes lethal suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells the function of the 26S proteasome is completely eliminated.
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