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code:02-060、02-060-5
Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.
Applications
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA (1 2)
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT) (3)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA (4)
Specifications
Form: 50 units/ul in 20mM Tris-HCl (pH 7.5) 100mM KCl 1mM DTT 50% Glycerol
Specific Activity: 100000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃.
Storage: at -20℃
Quality Assurance: Greater than 95% protein determined by SDS-PAGE (CBB staining) (Fig.1). Endo- and exo-DNase activities and RNase activity were not detected with 100 U/ml RNaseH in 50 ul reaction at 37℃.
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