anti-E.coli RuvA antibody rabbit serum

code:61-005

E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between (1 2).
Using this antiserum in Western blotting the band of 22kD corresponding to RuvA was obtained from the extract of E. coli cells (Fig.1).

Applications
1) ELISA
2) Western blotting (x 3000 dilution) (Fig.1)
Other applications have not been tested.

Immunogen: Purified full-size recombinant RuvA protein (2)
Form: 　antiserum added with 0.05% sodium azide
Storage: -20℃ for short period (about a half year)
For longer period store at -80℃

anti-E.coli RuvB antibody rabbit serum

code:61-007

E. coli RuvB protein forms a complex with RuvA protein and the complex promotes branch migration of Holliday junction at the late stage of homologous recombination and recombination repair. RuvB is a DNA motor protein which possesses the ATPase activity activated by DNA and RuvA protein (1 2). Its molecular weight is 37kD.

Applications
Western blotting (x 3000 dilution Fig.1)
Other applications have not been tested.

Immunogen: Purified full-size recombinant RuvB protein (Ref. 2).
Form: antiserum added with 0.05% sodium azide
Storage: -20℃ for short period (about a half year)
For longer period store at -80℃

anti-E.coli RuvC antibody rabbit serum

code:61-009

E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure an intermediate of recombination at the late stage of homologous recombination and recombinatioin repair and introduces nicks at the symmetrical points of the Holliday junction cleaving and resolving the recombinant (1). Its molecular weight is 19kD and forms a dimmer in liquid.

Applications
Western blotting for the detection of E. coli RuvC protein (x 3000 dilution Fig.1)
Other applications have not been tested.

Immunogen: Purified full-size recombinant RuvC protein (Ref. 2 3)
Form: rabbit antiserum added with 0.05% sodium azide
Storage: -20℃ for short period (about a half year)
For longer period store at -80℃

anti-Rad21(human、hRad21)antibody、rabbit antibody

Code:70-105

Rad21 (631 aa 71 kDa) 2) is a cleavable component of cohesin complex involved in chromosome cohesion during cell cycle in DNA repair and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition this protein is cleaved by separase/ESPL1 and dissociates from chromatin allowing sister chromatids to segregate.

anti-E.coli UmuD antibody rabbit polyclonal antiserum

code:61-011

The products of umuD umuC and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor LexA protein in uninduced cells (2). Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes umuD C and recA. UmuD protein is then auto-cleaved which is promoted by RecA protein ssDNA in a ATP-dependent manner (1). The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as a error-prone translesion DNA polymerase (3).
The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14KD (1 & Fig.1).

Application
Western blotting (x 3000 dilution Fig.1)
Immunogen: Purified recombinant LacZ’-UmuD fusion protein
Form: antiserum added with 0.05% sodium azide
Storage: -20℃ for short period (about a half year)
For longer period store at -80℃

Anti-E. coli DNA polymerase 1 antibody rabbit antiserum

Code:61-012

Key Words: DNA polymerase 1 polA A-family DNA polymerase DNA replication DNA repair Proof reading 3-5 exonuclease activity 5-3 exonuclease activity

Function: E. coli DNA polymerase 1 (928 aa; 103 kDa) is encoded by polA gene and involved in DNA replication and repair. In addition to polymerase activity this DNA polymerase exhibits 3 to 5 and 5 to 3 exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.