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Code:65-101
Background:A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by infecting mouse with SARS virus and as the clone which produces the antibody that specifically reacts with the virus-infected cell in the laboratory of Prof. K. Ikuta of Osaka University. Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.
Applications
1) Western blotting (0.1~0.3 μg/ml) 2) Immunofluoresece
staining (IHC) 3) ELISA
Isotype: IgG2b (kappa)
Form: Purified IgG 1 mg/ml in PBS (-) 50% glycerol filter-
sterilized azide free
References: This product has been used in references 2.
1. Satija N Lal SK The molecular Biology of SARS coronavirus. Ann. N.
Y. Acad. Sci. 1102 26-38 (2007)
2. Yamate M. et al. Establishing of Vero E6 cell clones persistently
infected with severe acute respiratory syndrome coronavirus.
Microbes and Infection 7:1530-1540 (2005)
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