HIV-1 Reverse Transcriptase active

Difinition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37℃.
Conditions of measurement: 50 mM Tris-HCl (pH 8.3) 10 mM MgCl2 50mM KCl 3 mM DTT 0.1% Nonidet P-40 20 g/ml poly(rA)・oligo(dT)12-18 　　0.5 mM dTTP(([3H]dTTP ~1 x 105 cpm) and 10-50 units/ml reverse transcriptase.
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.5 mg/ml as measured by BCA method
Specific activity: 5000 units/ml
Form: 50% glycerol 40 mM Tris-HCl (pH8.3) 50 mM NaCl 5 mM MgCl2
0.1% Triton X-100 1mM DTT
Storage: -20℃

HIV-1 Gag P17

Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.23 mg/ml as measured by BCA method
Form: 50% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM mercaptoethanol
Storage: -20℃

HIV-1 Gag P24

Full length recombinant HIV-1 type B Gag p24 protein expressed and proteolytically processed in E. coli in a soluble form without any tag attached.

Applications.
1. ELISA
2.Western blot as antigen
3. SDS-PAGE

Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 1 mg/ml as measured by BCA method
Form: 50% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM mercaptoethanol
Storage: -20℃

HIV-1 Gag P15

Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.42 mg/ml as measured by BCA method
Form: 50% glycerol 20 mM Tris-HCl (pH 7.5) 50 mM NaCl 10 mM mercaptoethanol
Storage: -20℃

HIV-1 Gag P55 active as HIV-1 protease substrate

05-009
05-010

Storage temperature : Ship with dry-ice or ice-pack and store at -20℃ (longer period-80℃)

Product: Recombinant full-size HIV-1 subtype 1、clone pNL4-3 (Ref 2) expressed in E. coli.

Applications
1) Substrate for the HIV-1 protease activity assay.
2) It can be used in detection of anti-HIV-1 Gag antibody in
Western blotting or ELISA. All the anti-HIV-1 Gag antibodies
such as anti-p17 antibody anti-p24 antibody and anti-p15
antibody can be measured at the same time.

Purity: Over 90% by SDS-PAGE (CBB staining)

Protein concentration: 0.5~1.0 mg/ml as determined by BCA method.

Form: 20% glycerol 20mM Tris-HCl (pH7.5) 50mM NaCl 10mM
mercaptoethanol

Background: HIV-1 Gag p55 is a precursor protein of several proteins that form the core structure of AIDS virus which are indispensable to their reproduction. This protein is digested by HIV-1 protease first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1 whose functions are unknown (1).

HIV-1 Nef

Code:05-011

Storage: Ship with ice-pack and store at-20℃.

Product: Full-size recombinant HIV-1 Nef protein expressed in
E.coli. No tag is attached.

Applications:
1) Functional studies of HIV-1 Nef protein
2) SDS-PAGE and Western blotting
3) ELISA

Purity: Over 90% by SDS-PAGE

Form: 50% glycerol 20 mM Tris-HCl (pH 7.5) 50 mM NaCl 10 mM
mercaptoethanol

Data Link: ProtKB: P03406 (NEF_HV1BR) GenBank: AAA44988.1

anti-HIV-1 Reverse Transcriptase antibody. guinea pig serum

Product: Polyclonal guinea pig antiserum raised against full-size recombinant HIV-1 reverse transcriptase
Applications: Western blotting. Immunoprecipitation. ELISA.IHC
Form: Undiluted antiserum added with 0.09% sodium azide .
Storage: Shipped at 4℃ or -20℃ and store at -20℃

anti-HIV-1 Gag p24 antibody. rabbit serum

Product: Polyclonal rabbit antiserum raised against intact recominant HIV-1 gag p24
Applications: Western blotting (X1000-3000) Immunoprecipitation. ELISA. IHC
Reactivity: Reactive with various substrains of HIV-1 p24 with little background noise. Reactive also with Gag precursor p55.
Form: Undiluted serum added with 0.09% sodium azide Storage:
Storage: Shipped at 4℃ or -20℃ and store at -20℃
Related Product: Recombinant HIV-1 Gag p24 (wothout tag)(#05-005. 006)
anti-HIV-1 gag p24 antibody (guinea pig serum) (#65-006)

Purified anti-p24 IgG solution without sodium azide is also available upon request

anti-HIV-1 Gag p24 antibody. guinea pig serum

Product: Polyclonal guinea pig antiserum raised against recombinant HIV-1 Gag p24 (no tag)
Applications: Western blotting (X1000-3000). Immunoprecipitation. ELISA.IHC
Reactivity: Reactive with various subtypes of HIV-1. Reactive also with Gag precursor p55
Form: Undiluted antiserum added with0.09% sodium azide
Storage: Shipped at 4℃ or -20℃ and store at -20℃

anti-HIV-1 p17 antibody. rabbit serum

Code:65-008

Product:Polyclonal rabbit antiserum raised against full-size recombinant p17 of HIV-1 (no tag)
Applications: Western blotting (X1000-3000). Immunoprecipitation. ELISA. IHC
Reactivity: Reacts with p17 as well as the gag precursors such as p55
Form: Undiluted serum added with0.09% sodium azide.
Storage: Shipped at 4℃ or at-20℃ and store at -20℃