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Code:02-300
Lambda Protein Phosphatase (Lambda-PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated serine threonine tyrosine and histidine residues. It is the 221 amino-acid product of ORF221 open reading frame on bacteriophage lambda (12). Lambda-PPase was expressed as a recombinant protein in E.coli and highly purified (2). This product is an intact enzyme of high quality without tag.
Applications:
Lambda-PPase can be used to release phosphate groups from phosphorylated serine threonine tyrosine and histidine residues in proteins (2). It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30℃. Inclusion of protease inhibitor cocktail and shortest incubation time is desired when assays are done with crude samples.
Form: 400 U/ul λ-PPase in 50mM HEPES (pH 7.5) 100mM NaCl 2mM dithiothreitol 0.1 mM MnCl2 0.1mM EDTA 50% glycerol 0.01% Brij 35.
Store at -20℃ for a week ( for longer period at -70℃ and avoid repeating of freezing- thawing)
Activity: 400 U/l where one unit is defined as the amount of enzyme that hydrolyzes 1 nmole of p-nitrophenyl phosphate per minute at 30℃. Unit definition assays are performed with 50mM p-nitrophenyl phosphate in Lambda-PPase buffer supplemented with 2 mM MnCl2 in a 50 ul reaction.
Specific Activity: 400000 U/mg
Quality Assurance:Greater than 95% homogeneous protein determined by SDS-PAGE (CBB staining) that contains no detectable protease activity
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