pEGFP-C2-RanBPM

Code:71-001-1

Full-sized Human RanBPM cDNA is fused to the C-terminus of EGFP (Enhanced Green Fluorescence Protein) on pEGFP-C2 vector (Clontech). It was constructed by Prof. T. Nishimoto. After his retirement it will be sent to academic colleagues upon request by BioAcademia. It will be charged $200 (or 20000 yen) for amplification maintenance and packaging.
Applications: By introducing the plasmid into mammalian cells RanBPM is expressed as a fusion protein with FEGF. It is useful to study the localization of RanBPM in cell compartment and interactions with functionally related proteins.

Protein Size Markers (Prestained) 12-270 kDa 50 times

Code:02-440

Prestained Protein Ladder Broad Range (12-270 kDa) is a mixture of highly purified proteins covalently coupled to a blue dye that resolves into 8 bands when electrophoresed. The covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins. The apparent molecular weights of the Prestained Protein Ladder bands were determined on 10–20% Tris-glycine SDS-PAGE gels by comparison to the Protein Ladder (BioAcademia 02-460 Protein Size Marker). The Prestained Protein Ladder is suited for use in SDS-PAGE and western blotting applications. It allows continuous monitoring of protein separations during electrophoresis and also provides a quick and easy way to assess blotting efficiency.

Protein Size Marker (Unstained) 12-250 kDa 50 times

Code:02-460

Protein Ladder Broad Range (12-250 kDa) is a mixture of highly purified proteins that resolves into 9 bands when electrophoresed. The Protein Ladder is suited for use in SDS-PAGE.
USAGE: Use 5 ul per lane in SDS-PAGE
Storage Temperature: -20°C
Solution: 70 mM Tris-HCl 33 mM NaCl 40 mM DTT 1 mM Na2EDTA 30% Glycerol
2% SDS pH 6.8 at 25°C