Taq DNA polymerase (+dNTPs) with Standard buffer

02-001 02-001-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase) has
thermostable DNA polymerase activity is suitable for PCR reactions.

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine)
at the 3’-blunt ends

Form;20mM Tris-HCl (pH 8.0) 100mM KCl 0.1mM EDTA 1mM DTT 50% glycerol
0.5% Tween20 0.5% Igepal CA-630.

Store at -20℃
Concentration:5 units/ul

Taq DNA polymerase (-dNTPs) with Standard buffer

02-011 02-011-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase) has thermostable DNA polymerase activity and suitable for PCR.

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine)
at the 3’-blunt ends

Storage Conditions:Ｓｈｉｐ at 4℃ or -20℃ and store at -20℃

Form: 20mM Tris-HCl (pH 8.0) 100mM KCl 0.1mM EDTA 1mM DTT 50% glycerol
0.5% Tween20 0.5% Igepal CA-630 Store at -20℃

Concentration: 5 units/ul

Pfu DNA polymerase (+dNTPs) Economy with reaction buffer

Code:02-021、02-021-5

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa same as that of the natural Pfu DNA polymerase.
■ Pfu DNA polymerase is thermostabe and has low error rates.
■ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
■　Pfu DNA polymerase-generated PCR
fragments are blunt-ended.
Applications:
1) cloning
2) DNA expression
3) site-directed mutagenesis
Storage Conditions:
50mM Tris-HCl (pH 8.2) 0.1mM EDTA 1mM DTT 50% glycerol 0.1% Tween20 0.1% Igepal CA-630 Store at -20℃
Concentration:2.5 units/l where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.

Pfu DNA polymerase (-dNTPs) Economy with reaction buffer

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa same as that of the natural Pfu DNA polymerase.
■ Pfu DNA polymerase is thermostabe and has low error rates.
■ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
■　Pfu DNA polymerase-generated PCR
fragments are blunt-ended.
Applications:
1) cloning
2) DNA expression
3) site-directed mutagenesis
Storage Conditions:
50mM Tris-HCl (pH 8.2) 0.1mM EDTA 1mM DTT 50% glycerol 0.1% Tween20 0.1% Igepal CA-630
Store at -20℃
Concentration:2.5 units/l where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.

Taq DNA polymerase Economy　(+dNTPs) with Enhancer for High GC template　and Robust buffer

02-003 02-003-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase)
thermostable DNA polymerase activity. This enzyme kit is especially
suitable for PCR reactions with high GC template due to Ehancer for high
GC templates and Robust buffer.
Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide
(adenosine) at the 3’-blunt ends (for cloning into TA vector)

Taq DNA polymerase Economy (-dNTPs) with Robust Buffer

02-012 02-012-5

Thermus aquaticus DNA polymerase (Taq DNA polymerase) has thermostable DNA polymerase activity and is suitable for PCR reactions

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine)
at the 3’-blunt ends for cloning into
TA vector.

Taq DNA polymerase Economy　(-dNTPs) with Enhancer for High GC template　and Robust buffer

Thermus aquaticus DNA polymerase has thermostable DNA polymerase
activity and this kit is especially suitable for PCR reactions
with high GC template due to Ehancer for high GC templates and
Robust buffer.

02-013 02-013-5

Applications:
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP dITP and
fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide
(adenosine) at the 3’-blunt ends (for
cloning into TA vector)

Taq Premix

code:02-100、02-100-5

Taq Premix is an optimized ready-to-use solution containing Taq DNA Polymerase dNTPs MgCl2 KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions bacterial colonies and cDNA products.

Taq Premix with Dye

code:02-102、02-102-5

Taq Premix with Dye is an optimized ready-to-use solution containing Taq DNA Polymerase dNTPs MgCl2 KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions bacterial colonies and cDNA products.

Taq Blend with Pfu (+ dNTPs)

Code:02-120、02-120-5

Taq Blend with Pfu is optimized blend of Taq and Pfu DNA polymerases. The proof-reading 3’→5’ exonuclease activity of Pfu increases the fidelity and robust amplification of Taq DNA polymerase. The reaction buffer has been formulated for robust yields and long PCR.