anti-CDC6 antibody rabbit polyclonal affinity-purified

Code:70-133

Storage: Sent at 4℃ and upon arrival spin-down and store at -20℃

Reactivity: human mouse rat

Immunogen: Recombinant GST-human CDC6 (1-326 amino acids) expressed in E. coli

Validation: Specificity of reaction has been validated with siRNA in WB (Ref.5 & 7)

Applications:
1. Western blotting (1/1000~3000)
2. Immunoprecipitation　（1/200）
3. Chromatin Immuno-Precipitation (assay dependent)
4. Immunofluorescence staining (1/200)

Form: 1 mg/ml in PBS with 50% glycerol. Filter-sterilized. Azide and carrier free

Function: CDC6 (Cell Division Control Protein 6 homolog) (human; 560 aa 62.7 kDa) is involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular location: The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Purification: The antiserum was first adsorbed with GST conjugated agarose column and
then the antibody in the pass-through fractions were purified with GST-CDC6 conjugated column.

Data base Link: uniprot/Q99741 (CDC6_HUMAN)

anti-Brg1 antibody rabbit polyclonal affinity-pure

Code:70-230

Key words: BAF complex Chromatin remodeling complex CREBBP CREST-BRG1 complex CYP27B1 gene c-Fos promoter Nuclear hormone receptor Neural stem cell Neuron NR2B promoter phospho-RB1-HDAC repressor complex Transcriptional coactivator Vitamin D receptor WINAC complex Zeb1

Applications
1) Western blotting (1/1000 dilution) (Fig. 1)
2) Immunoprecipitation (Assay dependent) (Fig. 2)　
3) Immunofluorescence staining (1/1000 dilution) (Fig. 3)
Not tested for other applications.

anti-Nup62 antibody rat monoclonal

Code:70-305

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .

Applications
1. Western blotting (1/500 ~1/2000 dilution)
2. Immunoprecipitation (assay dependent)
3. Immunofluorescece / Immunocytochemistry (1/400)
4. ELISA (assay dependent)
5. When this antibody was micro-injected into the cytoplasm of the HeLa cells it accumulates into the nuclear pores as examined by immunofluorescence staining..

anti-EDD/UBR5 antibody rabbit polyclonal

Code：70-501

EDD / UBR5 (2799 aa 309 kDa) is E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168 an E3 ubiquitin-protein ligase that promotes accumulation of Lys-63-linked histone H2A and H2AX at DNA damage sites thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.

Anti-SUMO 2/3 antibody rat monoclonal (3H12)

code:70-657

SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination which targets proteins for degradation SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport gene expression cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human; SUMO1 SUMO2 and SUMO3. SUMO2 and 3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO2/3 forms poly-(SUMO) chains is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Molecular mass: SUMO2; proform 10871 Da with 95 aa (94-95 aa are removed from proform). SUMO3; proform 11637 Da with 103 aa (93-103 aa are removed from proform).
Applications:
1. Western blotting (1/1000)
2. Immunocytochemistry/Immunofluorescence staining (1/100~1/500)
3. Immunohistochemistry frozen section (1/100~1/500)
4. ELISA (Assay dependent concentration)
Other applications have not been tested.

Anti-ACE3 antibody rabbit polyclonal

code:73-006

Key words: Angiotensin converting enzyme-like 3 Sperm Acrosomal cap IZUMO1 interacting protein
Function: ACE3 was identified as an IZUMO1 interacting protein from mouse sperm. ACE3 protein is exclusively localized in testis and sperm. However unlike the disruption of IZUMO1 or ACE elimination of ACE3 in mouse did not result in loss of sperm fertilizing ability.
Molecular mass: ACE3 consists of 737 amino acids with molecular mass of 85874 Da.
Applications:
1. Western blotting (1/500 dilution)
2. Immunoprecipitation (1/100 dilution)
3. Immunofluorescence staining (1/100 dilution)
Other applications have not been tested.

Anti-Calreticulin / CALR antibody rabbit polyclonal

code:73-018

Key words: Calreticulin CALR XCRP55 Calregulin Endoplasmic reticulum resident protein 60 (ERp60)
Lectin chaperon calcium homeostasis
Function: Calreticulin (CALR) is calcium-binding chaperone that promotes folding oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis
Molecular mass: CALR consists of 416 amino acids with molecular mass of 47995 Da.
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100)
Other applications have not been tested.

Anti-Calreticulin-3 / CALR3 / Calsperin antibody rabbit polyclonal

code:73-022

Key words: Calreticulin-3 CALR3 Calsperin EIF2 complex Spermatogenesis Differentiation Endoplasmic reticulum
Function: CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. During spermatogenesis may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility.
Molecular mass: 44232 Da with 380 amino acids.
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100 dilution)
3. Immunohistochemistry (1/100 dilution)
Other applications have not been tested.

Anti-Calnexin antibody rabbit polyclonal

code:73-026

Key words: Canx Endoplasmic reticulum Chaperon Synaptic vesicle endocytosis Transmembrane Calcium ion binding

Function: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse
Molecular mass: 67278 Da with 591 amino acids
Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100dilution).
Not tested for other applications.

Anti-CD52 antibody rabbit polyclonal

73-030

Product: rabbit anti-CD52 antibody rabbit serum added with 0.09% azide

Storage: Shipped at 4℃ and stored at -20℃

Reactivity:mouse

Applications:
1.Western blotting (1/1000 dilution)
2.Immunofluorescent staining
3.Immunohistochemistry


Function: CD52 may play a role in carrying and orienting carbohydrate as well as having a more specific role. Expressed on lymphohematopoietic tissues including thymus spleen and bone marrow but not in liver kidney and brain.
Molecular mass: 7798 Da with 74 amino acids. In mature form propeptide is removed and GPI anchored and glycosylated.