Anti-POGZ antibody rabbit serum

Code:70-112

POGZ (pogo transposable　element-derived protein with zinc finger domain; 1410 aa 155 kDa) plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 (HP1 alpha) it plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.

Applications
1. Western Blotting (1/1000 dilution)
2. Immunofluorescece staining (1/500 dilution)
3. Immunoprecipitation (assay dependent)
Not tested for other applications

anti-VRK1(human) antibody mouse monoclonl (5D1)

Code:71-600

Function; The VRK1 gene encodes serine/threonine kinase VRK1 (Vaccinia-Related Kinase 1; 396 aa 45.5 kDa) which is involved in Golgi disassembly during the cell cycle following phosphorylation by PLK3 during mitosis and required to induce Golgi fragmentation. It acts by mediating phosphorylation of a downstream target protein Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. It also phosphorylates casein and histone H3. Phosphorylation of the BANF1 gene product disrupts its ability to bind DNA reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm.
Involvement in disease Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1A (PCH1A); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1A is characterized by an abnormally small cerebellum and brainstem central and peripheral motor dysfunction from birth gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy

Applications
1. Western blotting (1/200~1/1000 dilution).　Use of　highly sensitive chemiluminescence reagents such as Lumi-Light Plus (Roche) or ImmunoStar○LD (Wako Tokyo) are recommended.
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining (1/100 dilution)
4. Immunohistochemistry (assay dependent)
5. ELISA (assay dependent)

anti-CDT2/DTL antibody rabbit serum

70-115

Validation: Specificity was validated by western blotting with siRNA

Storage: Ship at 4℃ and aliquot and store at -20℃
Reactivity; Human and mouse rat hamster

Applications
1. Western blotting (1/200-1/2000 dilution).
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining (1/100~1/1000 dilution)


Immunogen ; E. coli expressed and purified 6×His-tagged C-terminal 150 amino acids of human Cdt2
Form; Rabbit polyclonal antiserum added with 0.05 % sodium azide

Function; CDT2 (human 730 aa 79.5 kDa) is substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control DNA damage response and translesion DNA synthesis. The DCX(DTL) complex also named CRL4(CDT2) complex mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication.

anti-5Methylcytosine antibody mouse IgM Biotinylated

Code:51-004

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA ― for example to the number 5 carbon of the cytosine pyrimidine ring ― which involves reduction in gene expression. In adult somatic tissues DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.

anti-5Methylcytosine antibody mouse IgM FITC-conjugated

Code:51-005

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA ― for example to the number 5 carbon of the cytosine pyrimidine ring ― which involves reduction in gene expression. In adult somatic tissues DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.

anti-Nup98 antibody mouse monoclonal (13C2)

Code:70-345

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

anti-Nup98 antibody mouse monoclonal (21A10)

Code:70-346

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

anti-Nup98 antibody mouse monoclonal (13C2+21A10)

Code:70-347

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In addition Nup98 plays roles in several important biological events such as gene expression mitotic checkpoint and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation.
In ciliates a unicellular organism having two functionally distinct nuclei GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus and these different Nup98s participate in a nucleus-selective transport mechanism.

anti-MCM7 antibody rabbit polyclonal IgG

Code:70-120

Key words: DNA replication licensing factor MCM complex DNA replication initiation G1/S
transition DNA damage response DNA helicase
Function: MCM7 (human; 718 aa 80 kDa) acts as component of the MCM2-7 complex (MCM complex)
which is the putative replicative helicase essential for once per cell cycle DNA replication initiation
and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through
the interaction surfaces of two neighboring subunits such that a critical structure of a conserved
arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the
adjacent subunit. The six ATPase active sites however are likely to contribute differentially to the
complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Applications
1) Western blotting (1/1000~1/5000 dilution).
2) Immunoprecipitation (assay dependent)
3) Immunofluorescence staining (1/200~1/1000 dilution).
Other applications have not been tested.

anti-GRWD1 antibody rabbit polyclonal affinity-pure

Code:70-130

Reactivity: Human mouse and rat. Other species have not been tested.

Validation: Validated for Western Blotting by siRNA.
Immunogen: Purified GST-GRWD1 (human full-length) expressed in E. coli.
Applications:
1. Western blotting (1/1000~1/3000 dilution)
2. Immunoprecipitation (Assay dependent)
3. Chromatin Immuno-Precipitation (Assay dependent)
4. Immunofluorescence staining / Immunochemistry (1/100~1/1000
dilution)