Anti-ATF6 alpha antibody mouse monoclonal (37-1)

code:73-505

ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress. When unfolded proteins accumulate in the ER ATF6 alfa is cleaved by regulated intramembrane proteolysis. The resulting amino-terminal fragment translocates to the nucleus and activates transcription by binding to ER stress-response elements present in the promoter regions of ER stress-inducible genes including those encoding ER chaperones and components of ER-associated degradation. ATF6 consists of two closely related factors ATF6 alfa and ATF6 beta in mammals. ATF6 alfa but not ATF6 beta plays a pivotal role in transcriptional control.
The monoclonal antibody was characterized in the laboratory of Professor Kazutoshi Mori of Kyoto University. The antibody was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Applications: (Detailed Protocol is given below)
1. Western blotting
2. Immunoprecipitation (IP) (less efficient than clone1-7)
This antibody does not work for immunofluorescence analyses.
Immunogen: Recombinant ATF6 (amino-terminal fragment of ATF6 fused to GST)
Isotype: mouse IgG1 Epitope: not determined
Form: purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized
Specificity: Reactive to human and mouse ATF6However clone 1-7 antibody (#73-500) is recommended for human cells.
Storage: Shipped at 4℃ or -20℃ and stored at -20℃
Data Link Swiss-Prot P18850 (human ATF6 alpha)
References: This antibody is described in Ref 1.
1.Mori K “Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!”Mol Biol Cell 21: 11435-8 (2010) PMID:20219975

anti-Nestin(mouse、rat) antibody rabbit serum

Code:73-105

Shipping and Storage: Shipped at 4℃. Store at-20℃. Do not freeze.

Nestin is a class VI intermediate filament protein that is abundantly expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Upon differentiation nestin becomes down-regulated and is replaced by other intermediate filament proteins. Nestin expression is widely used as a marker for CNS stem cells in the developing nervous system. Its transient expression is a critical step in the neural differentiation pathway.

Applications:
1. Western blotting (dilution: 1/500-1/5000)
2. Immunocytochemistry (dilution: 1/300-1/1000)
3. Immunohistochemistry (dilution: 1/500-1/1000) Not tested for other applications

Immunogen: Synthetic peptide corresponding to the C-terminal 15 aa of mouse nestin.

Specificity: Reacts with mouse and rat Nestin but not with human Nestin.
　　
Form: Protein A-purified IgG 2 mg/ml in PBS 50% glycerol. Filter-sterilized. Carrier and azide free.

Data Link: Swiss-Prot Q6P5H2 (mouse) P21263 (rat)

anti-Caspase 3 activated (p20/p17 subunit; human、rat、mouse) antibody rabbit serum

Code:74-102

Caspases are a family of cysteine proteases which play essential roles in apoptosis. Among them Caspase 3 is a frequently activated death protease catalyzing the specific cleavage of many key cellular proteins. Caspase 3 is synthesized as an inactive 32 kDa pro-enzyme which undergo proteolytic processing in response to apoptotic stimulation to produce the active form which consists of the p20/p17 and p12 subunits. Caspase 3 is the predominant caspase involved in the cleavage of Alzheimer amyloid precursor protein (APP) which is associated with neuronal death in Alzheimer ‘s disease. An antibody (named ACP3) against activated caspase 3 was raised in rabbit. This antibody recognizes the active form of human caspase 3 p20/p17 subunit but does not recognize the proenzyme p32.

anti-Amyloid Precursor Protein (APP C-terminus; human、mouse、rat) antibody rabbit serum

Code:74-104

The Alzheimer amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known though it has been implicated as a regulator of synapse formation and neural plasticity. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates amyloid beta (A) a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer’s disease patients. Isoform APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. An antibody (named AC1) against the C-terminus of human APP695 (aa 671-695) was raised in rabbit (2).

anti-Amyloid Precursor Protein (APP N-terminus; human、mouse、rat) antibody rabbit serum

Code:74-106

The Alzheimer amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known though it has been implicated as a regulator of synapse formation and neural plasticity. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates amyloid beta (A) a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer’s disease patients. Isoform APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. An antibody (named AN2) against the N-terminus of human APP (aa 18~38) was raised in rabbit.

anti-APP-C31 (C-terminal fragment of the caspase 3-cleaved APP; human、mouse、rat) antibody rabbit serum

Code:74-108

The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APPC31) and the 31-residues C-terminal fragment (APP-C31). Both fragments might be potent inducers of neuronal apoptosis. An antibody (named ACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of human APP695 (APP-C31) was raised in rabbit.

anti-APP ⊿C31 (specific to C-terminal APP ⊿31; human、mouse、rat) antibody rabbit serum

Code:74-110

The Alzheimer amyloid precursor protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APP delta C31) and the 31-residues C-terminal fragment (APP-C31). APP delta C31 potentially plays pathophysiological roles in neuronal death (ref.3). An antibody (named SAC) against the C-terminus of caspase 3-cleaved human APP695 (APP delta C31) was raised in rabbit.

anti-MAGE-D1/Dlxin-1/NRAGE antibody rabbit serum

Code:74-112

MAGE-D1 (melanoma-associated antigen D1) also known as Dlxin-1 or NRAGE is a member of the MAGE protein family. MAGE-D1 is expressed in almost all normal adult tissues and has been demonstrated to interact with the p75 neutrophin receptor and to facilitate nerve growth factor-dependent apoptosis (ref.1). MAGE-D1 also interacts with its homologous protein necdin which is known as a growth suppressor and a promoter of differentiation in neurons. Necdin binds to Msx (msh homeobox) and Dlx (distal-less homeobox) family homeodomain transcription factors via MAGE-D1. These proteins cooperate to modulate differentiation of neurons and muscle cells by regulating gene expression whereas Msx proteins function as transcriptional repressor and Dlx proteins function as transcriptional activators (ref.2 and 3).
An antibody (named MD1) against mouse MAGE-D1 was raised in rabbit (ref.2).

anti-MAGE-G1/Necdin-like 2 (human、mouse、rat) antibody rabbit serum

Code:74-114

MAGE-G1 (melanoma-associated antigen G1 also designated necdin-like 2) gene encodes a necdin homologous protein. MAGE-G1 gene similarly to necdin gene has been mapped to the region of proximal chromosome 15q in human which is subject to genomic imprinting and implicated in various human neurological and mental disorders (ref.1). From this finding it is suggested that MAGE-G1 is involved in brain development and its abnormality causes neurodevelopmental diseases although its biochemical and functional features remain largely unknown. MAGE-G1 has characteristics similar to those of necdin which suppresses cell growth by inducing cell cycle arrest. MAGE-G1 like necdin targets both the transcription factor E2F1 and p75 neutrophin receptor (p75NTR) to regulate cell viability during brain development (ref.2). An antibody (named MG1) against mouse MAGE-G1 was raised in rabbit (ref.2).

Anti-GFP antibody rabbit serum

code:60-011

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa) originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology the GFP fused gene is frequently used as a reporter of expression and protein localization (2 3).