anti-Nestin antibody. rat monoclonal(7A3)

73-100

Storage: Ship at 4℃ and store at -20℃

Immunogen: Mouse E16 embryonic cerebral cortex extracts

Applications
1. Immunocytochemistry
2. Immunohistochemistry
This antibody doesn’t work in immunoblotting. O

Isotype: Rat IgG2b κ

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS
50% glycerol filter-sterilized

Specificity: Specific to mouse Nestin not tested with other species.



Nestin is an intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Nestin is replaced in the adult organism by other intermediate filament proteins however it may be re-expressed under certain pathological conditions such as ischemia inflammation brain injury and neoplastic transformation. Nestin has been detected in many kinds of tumors especially in tumors derived from the CNS therefore it is considered to be a marker for cancer stem cells in neurogenic tumors.


References:. Iriuchishi H. Neovascular niche for human myeloma cells in immunodeficient mouse bone
PLoS One. 2012;7(2):e30557. PMID: 22347385 IHC-F (mouse)

anti-PHLDA3 antibody mouse monoclonal (4B6)

Code:71-195

PHLDA3 (Pleckstrin homology-like domain family A member 3) is a small PH (Pleckstrin homology) domain-only protein (127 a.a. in human) that is highly conserved in vertebrates. Recently PHLDA3 was found to be a p53-regulated repressor of Akt (ref 2) hence it is speculated as a tumor suppressor. PHLDA3 also inhibits anchorage-independent cell growth. PHLDA3 gene is frequently lost in human lung endocrine tumors.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

anti-Glyoxalase1 (GLO1、human、mouse) antibody; rat monoclonal (6F10)

Code:74-001

Glyoxalase I (GLO1) is an enzyme that plays a role in the detoxification of methylglyoxal (MG) a side-product of glycolysis via condensation with glutathione to produce S-lactoyl-glutathione. GLO1 is a zinc metalloenzyme whose crystal structure has been solved. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric and its sequence is well conserved. GLO1 is found over-expressed in some tumors. GLO1 has also been suggested to be involved in anxiety diseases autism and Alzheimer’s disease.
The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Applications
1. Western blotting (~X 1000) 2. Immunocytochemistry 3. ELISA
Other applications are not tested.

Immunogen: Recombinant GST-fused mouse glyoxalase I (full length)

Isotype: Rat IgG2b κ

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human simian and mouse glyoxalase I.
Other species are not tested.

References:
1. ChenF. et al. (2004) Role for glyoxalase I in Alzheimer’s disease.” Proc. Natl. Acad. Sci. USA 101: 7687–7692.
2. Junaid M.A. et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J. Med. Genet. A. 131: 11–17.
3. Hovatta I. et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice Nature 438: 662–666.

Anti-GFP antibody rat monoclonal (1A5)

60-001

Storage: Shipped at 4℃ and store at -20℃.

Reactivity: Reacts with GFP EGFP and their fused proteins
Immunogen: Recombinant GFP protein

Applications
1. Western blotting (~1ug/ml) 　2. Immunoprecipitation 　3. Immunocytochemistry
4. Chromatin Immunoprecipitation (ChIP) 　 5. ELISA

Isotype: Rat IgG1 kappa
Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50%
glycerol filter-sterilized Azide and carrier free.

Background: The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa) originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light .　 In cell and molecular biology the GFP fused gene is frequently used as a reporter of expression and protein localization .

Anti-Sup35/PSI+ (S.cerevisiae) antibody rabbit polyclonal IgG

62-300

Storage: Ship at 4℃ and store at -20℃

Reactivity: S. cerevisiae Sup35 not tested with other species

Immunogen: Synthetic peptide corresponding to a.a. 494-507 of Sup35

Applications:1) Western blotting (1000~2000 fold dilution)

Product: Rabbit polyclonal antibody purified IgG fraction

Form: 　 Purified IgG 4 mg/ml in PBS 50% glycerol 0.09% sodium azide

Background: Sup35 protein of S. cerevisiae is the translation termination factor eRF3. The altered conformation of this protein generates the [PSI+] prion phenotype . In this state a dominant cytoplasmically inherited protein aggregates are formed which sequester the normal function of Sup35 thereby nonsense suppressor phenotype is created . The molecular chaperon Hsp104 is necessary for the formation and maintenance of the aggregates .
　

anti-Rnq1 (S. cerevisiae) antibody affinity purified

Code:62-301

Shipping and Storage temperature: Ship with ice-pack and store at -20℃. Do not freeze.

Reactivity: S. cerevisiae Rnq1 not tested with other species

Applications: Western blotting (1/1000). Not tested for other applications.

Immunogen: Synthetic peptide CSQQNNNGNQNRY corresponding to the C-terminus region of Rnq1

Product: Rabbit polyclonal antibody affinity-purified with protein A column.

Form: 1 mg/ml in PBS 50% glycerol. Filter-sterilized. No additive.

anti-Cdc37 (S. cerevisiae) antibody rabbit serum

Code:62-302

Background: Cdc37 was initially identified as a cell division cycle control protein of Saccharomyces cerevisiae (1) and was later found to have a much broader role as a molecular chaperone required for folding of protein kinases (2). It forms complex with Hsp90 and a variety of protein kinases and is thought to play a critical role in directing Hsp90 to its target kinases (3). Cdc37 has a molecular weight of 58.4 kD.

Anti-Dnmt3b antibody rabbit polyclonal affinity-pure

code:70-206

Dnmt3b (DNA (cytosine-5-)-methyltransferase 3b) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics).　Dnmt3b is thought　to function in de novo methylation rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene　cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
This antibody was prepared and characterized by Prof. S. Tajima of Osaka Univ. and used in Ref.2~4.
Applications
1) Western blotting (1000-fold dilution)
2) Immunoprecipitation (1000-fold dilution) useful for ChiP assays
3) Indirect immuno-fluorescence staining (1000-fold dilution)
4) Immunohistochemistry (1000-fold)
Properties of the product
Immunogen: Highly purified recombinant mouse Dnmt3b (amino acids 1-181) (accession no. AF068626）
Antibody: Affinity-purified with the recombinant Dnmt3b
Reactivity: Mouse rat and human
Form: 1 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)
Storage: -20℃ (long period; -80℃)

Anti-ATF6 alpha antibody mouse monoclonal (1-7)

73-500

Applications:
1. Western blotting
2. Immunoprecipitation (IP)
3. immunofluorescence staining.

Immunogen: Recombinant ATF6 alfa; (His-tagged amino-terminal fragment of ATF6 alfa;)


Isotype: mouse IgG2a kappa;

Form: purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: specific to human ATF6 alfa no cross reactivity with mouse ATF6 alfa;




ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress. When unfolded proteins accumulate in the ER ATF6 alpa; is cleaved by regulated intramembrane proteolysis. The resulting amino-terminal fragment translocates to the nucleus and activates transcription by binding to ER stress-response elements present in the promoter regions of ER stress-inducible genes

The monoclonal antibody was characterized in the laboratory of Professor Kazutoshi Mori of Kyoto University.

anti-Necdin(human、mouse、rat) antibody rabbit serum

74-100
Storage: Ship at 4℃ and store at -20℃. (No not store below -20℃)

Validation: Specificity of reaction has been validated with knock-out mice by western blot and IHC-F

Reactivity: React with mouse rat human chicken

Immunogen: Recombinant GST-fused mouse necdin (aa 83-325)
.
Applications:
1. Western blotting (1/1000-1/3000)
2. Immunohistochemistry、frozen section (1/500)
3. Immunocytochemistry (1/500)
4. Immunoprecipitation (1/100)
5. Chromatin Immunoprecipitation (1/100)
6.Immunoaffinity assay (Identification of Necdin interacting proteins by column conjugated with anti-Necdin antibody)

Form: Protein A affinity purified IgG. 1 mg/ml in PBS 50% glycerol. Filter-sterilized. No additive.