Anti-Taf14 (S. cerevisiae) antibody rabbit polyclonal

Code:62-025

Storage: Shipped at 4℃and stored at -20℃ for long period.

Immunogen: Recombinant His-tagged Taf14 protein (full-length; 1-244 aa)
produced in E. coli

Reactivity: S. cerevisiae Taf14 protein. Not tested with other species

Applications: Western blotting (1/500-1/2000). Not tested for other
applications.

Form: Whole antiserum added with 0.1% sodium azide


Background: Taf14 functions as a component of the DNA-binding general
transcription factor complex TFIID the RNA polymerase II associated
general transcription factor complex TFIIF and the chromatin-remodeling
complex SWI/SNF.
Taf14 consists of 244 amino acids with molecular mass of 27440 Da

Anti-Tfa1 / TFIIEa (S. cerevisiae) antibody rabbit polyclonal

62-026
Storage: Shipped at 4℃and stored at -20℃ for long period.

Immunogen: Recombinant His-tagged Tfa1 protein (1-482 aa) produced in E. coli

Reactivity: S. cerevisiae Tfa1 protein. Not tested with other species

Applications: Western blotting (1/2000). Not tested for other applications.

Form: Whole antiserum added with 0.1% sodium azide

Background: Tfa1 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase
Taf14 consists of 482 amino acids with molecular mass of 54742 Da

Anti-Ada2 (S. cerevisiae) antibody rabbit polyclonal

62-027

Storage: Shipped at 4℃and stored at -20℃ for long period.

Immunogen: Recombinant His-tagged Ada2 protein (full-length; 1-434 aa)
produced in E. coli

Reactivity: S. cerevisiae Ada2 protein. Not tested with other species

Applications:
Western blotting (1/500-1/1000).

Form: Whole antiserum added with 0.1% sodium azide

Background: Ada2 functions as component of the transcription regulatory
histone acetylation (HAT) complexes SAGA SALSA and ADA. SAGA acetylates
nucleosomal histone H3 to some extent (to form H3K9ac H3K14ac H3K18ac
and H3K23ac). SAGA interacts with DNA via upstream activating 　
　sequences　 (UASs). ADA preferentially acetylates nucleosomal histones
H3 (to form H3K14ac and H3K18ac) and H2B　Ada2 consists of 434 amino
acids with molecular mass of 50569 Da.

Anti-Ada3 / Ngg1 (S. cerevisiae) antibody rabbit polyclonal

Code:62-028

Ngg1 transcription regulator. Could inhibit GAL4 DNA-binding or its ability to activate transcription. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA SALSA SLIK and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 SPT8 and SPT20) and promoter selectivity interaction with transcription activators (GCN5 ADA2 ADA3 and TRA1) and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac H3K14ac H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA an altered form of SAGA may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3 at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (at Lys-14 and Lys-18) and H2B.
NGG1 consists of 702 amino acids with molecular mass of 79282 Da

Anti-Med9 / Cse2 (S. cerevisiae) antibody rabbit polyclonal

Code: 62-029

Med9 is a component of the Mediator complex a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex having a compact conformation in its free form is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins
Med9 consists of 149 amino acids with molecular mass of 17376 Da

Anti-Med11 (S. cerevisiae) antibody rabbit polyclonal

Code:62-030

Storage: Shipped at 4℃ and stored at -20℃

Immunogen: Recombinant His-tagged Med11 protein (1-132) produced in E. coli

Reactivity: Reacts with S. cerevisiae Med11. Not tested with other species
.
Applications: Western blotting (1/100-1/200). Not tested for other applications.
Form: Protein A affinity purified IgG 2 mg/ml in PBS 50% glycerol

Background: Component of the Mediator complex a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. The Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential MED11/22 heterodimer specifically functions in promoting stable PIC formation

Data Link UniProt Q99278 (MED11_YEAST) SGD S000004718. MED11.

Anti-Rfa1/Rpa1 (S. cerevisiae) antibody rabbit polyclonal

Code:62-150

Background: Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A) a single-stranded DNA-binding heterotrimeric complex may play an essential role in DNA replication recombination and repair. Binds and stabilizes single-stranded DNA intermediates preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

Anti-Rfa3/Rpa3 (S. cerevisiae) antibody rabbit polyclonal

Code:62-152

Background: Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication repair recombination; RPA protects against inappropriate telomere recombination and upon telomere uncapping prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
RFA3 encodes 122 amino acids with molecular mass of 13816

Anti- PCNA (S. cerevisiae) antibody rabbit polyclonal ChIP grade

Code:62-160

Storage: Shipped at 4℃and store at -20℃ (avoid freezing by storing below -25℃)

Reactivity: Saccharomyces cerevisiae

Immunogen: Recombinant full-size PCNA (S. cerevisiae 1-258 aa)
Applications:
1. Western blotting (1/1000dilution)
2. Immuno-precipitation (1/100)
3. Chromatin Immuno-Precipitation (Assay dependent)

Form: Protein-A affinity purified IgG 2 mg/ml in PBS 50% glycerol. Filter sterilized. No preservative nor carrier protein added.

Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand. Involved in DNA repair and recombination. It consists of 258 amino acids with MW of 28916

Data Link: UniProt P15873 (PCNA_YEAST)

Anti- RRM1 / RNR-R1 antibody rabbit polyclonal

Code:69-100

Storage: Ship at 4℃ and store at -20℃

Reactivity: Xenopus human mouse rat hamster

Applications:
1. Western blotting ( 1/1000-1/2000 dilution)
2. Immunofluorescence staining (1/100 dilution)
Immunogen: Recombinant His-tagged RRM2 (182-717 amino acids) of Xenopus laevis.

Form: IgG (Protein A affinity purified) 2 mg/ml in PBS 50% glycerol Filter-sterilized

Background: RRM1 (Ribonucleoside-diphosphate reductase 1) catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides and provides the precursors necessary for DNA synthesis. RRM1 consists of 797 amino acids with molecular mass of 90833.
Data Link UniProtKB/Swiss-Prot Q6GM88 (Q6GM88_XENLA) Reference : This product was used in the following publication.
Takada S. et al. Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts. Mol Biol. Cell 11: 41734187 (2000) PMID: 11102516 WB IF (Xenopus)