anti-Nup98 antibody rat monoclonal (2H10)

Code:70-310

Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC) which is a large protein assembly embedded in the nuclear envelope. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 plays a specific role in the RNA export. Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation. This hybridoma has been established by Prof. T. Tachibana’s Lab. at Osaka City Univ.

Applications:
1) Western blotting (Fig. 1) 2) Immunocytochemistry (Fig. 2) 3)ELISA　4) Dot blotting

anti-HP1α/CBX5 antibody (rabbit) affinity-purified

Code:70-221

Backgrouds:Heterochromatin protein 1 (HP1) is a major component of heterochromatin and play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved and there are multiple members within the same species. In humans HP1α is encoded by the Chromobox homolog 5 (CBX5) gene. HP1α has been observed to interact with several non-histone proteins with a wide variety of functions (1).
The product is prepared by immunizing rabbit with the and by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.

Applications
1) Western blotting (1/1000~1/3000) (Fig.1)
2) Immunofluoresece staining

Immunogen: A synthetic peptide CEDAENKEKETAKS corresponding to the
amino acid sequence 179-191 of human HP1α
Antibody: Polyclonal rabbit anibodies purified with the peptide used
as the immunogen.
Reactivity: Human. Not tested with other species
Form: Purified IgG 0.37 mg/ml in 0.12 M sodium phosphate buffer (pH
7.4) 50% glycerol filter-sterilized azide free


References
1. Lomberk G et al. Genome Biol. 7 228 (2006) review
2. Kametaka A et al. Genes Cells 7 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26 4028 (2006)

Related product: 70-223 Anti-HP1βantibody 70-225 Anti-HP1γ antibody

anti-HP1β/CBX1 antibody (rabbit) affinity purified

Code:70-223

Backgrounds: Heterochromatin protein 1 (HP1) is a major component of heterochromatin　play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved and there are multiple members within the same species. In humans HP1β is encoded by the Chromobox homolog 1 (CBX1) gene. HP1βhas been observed to interact with several non-histone proteins with a wide variety of functions (1).
The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1β(3) and by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi (Ref. 3).
Applications
1) Western blotting (1/2000~1/10000) (Fig. 1)
2) Immunofluoresece staining (Ref. 3)
3) Chromatin immunoprecipitation (ChIP)(Ref. 3)
Reactivity: Human.Expected to react with mouse hamster and Drosophila
orthologs due to the sequence identity of the immunogen.
Form: IgG 0.44 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4)
50% glycerol filter-sterilized azide free

References: This product was used in reference 3.
1. Lomberk G et al. Genome Biol. 7 228 (2006) review
2. Kametaka A et al. Genes Cells 7 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26 4028 (2006)

Related products: Anti-HP1α antibody 70-225 Anti-HP1γ antibody

anti-HP1γ/CBX3 antibody (rabbit) affinity purified

Code:70-225

Backgrouds: Heterochromatin protein 1 (HP1) is a major component of heterochromatin　play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved and there are multiple members within the same species. In humans HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene . HP1γhas been observed to interact with several non-histone proteins with a wide variety of functions (Ref 1).
The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ(Ref 2 3) and by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.
Applications
1) Western blotting (1/2000~1/10000) (Fig. 1 & Ref 2)
2) Immunofluoresece staining (Ref. 2 & 3)
3) Chromatin immunoprecipitation (ChIP) (Ref. 3)
Reactivity: Human and hamster. Expected to react with mouse chicken
Xenopus Zebra fish and Drosophila orthologs due to the sequence
identity of the immunogen.
Form: IgG 0.75 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4) 50%
glycerol sterile-filtered azide free

References: This product was used in references 2 and 3.
1. Lomberk G et al. Genome Biol. 7 228 (2006) review
2. Kametaka A et al. Genes Cells 7 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26 4028 (2006)

Related product: 70- Anti-HP1α antibody 70-225 Anti-HP1β antibody

anti-5Methylcytosine antibody mouse IgM (clone 5MC-CD)

code:51-003

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. As such it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a methyl group to DNA ― for example to the number 5 carbon of the cytosine pyrimidine ring ― in this case with the specific effect of reducing gene expression. In adult somatic tissues DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
Applications　
1) Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
2) Immuno-blotting detection of DNA with 5-methylocytosine on nitrocellulose (Ref. 3 & 4) (~1000
fold dilution).
Immunogen: 5-Methylcytosine conjugated to bovine serum albumin (Ref 3)
Reactivity: DNA with 5-Methylcytosine (methylated DNA) any species
Isotype: IgM
Form: Purified mouse IgM 1 mg/ml in PBS with 50% glycerol filter-sterilized
Storage: -20℃ (long period -80℃)

anti-KDM5A/RBP2/JARID1A antibody mouse monoclonal (9A6)

Code:71-175

RBP2 (1690 aa 192 kDa) was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji AT rich interactive domain 1A) and KDM5A (gene name). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase.

anti-RBP2/JARID1A antibody mouse monoclonal (18E8)

Code:71-177

RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase (1 2 & 3).

Anti-Dnmt1 (1037-1386) antibrabbit polyclonal) ody affinity-purified (

Code:70-203

Dnmt1 (DNA (cytosine-5-)-methyltransferase 1) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Hypermethylation in promoter regions is asssociated with repression of gene expression. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1 2). This antibody was raised by Prof. S. Tajima of Osaka Univ. (3 4)
Applications
1) Western blotting (0.2～1 ug /ml)
2) Immunoprecipitation. Especially good for denatured protein and useful for ChiP assays
3) Indirect immuno-fluorescence staining
Properties of the product

Antigen: Recombinant mouse Dnmt1 (amino acids 1037-1386) purified by SDS-PAGE .

Antibody: Affinity-purified with the recombinant Dnmt1(1037-1386)

Reactivity: mouse human xenopus

Form: 1 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)

Storage: -20℃ (long period; -80℃)

Anti-EID1 antibody monoclonal (#2)

Code:71-190

EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation.

Anti-Nup153 antibody rat monoclonal (R4C8)

Code:70-315

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. This hybridoma has been established by Prof. T. Tachibana’s Lab at Osaka City Univ. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.

Applications
1. Western blotting (160 kDa single band in Hela cell extract) 2. Immunocytochemistry
3. ELISA
Other applications are not tested.
Additional comments: When injected into the nucleus R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well.

Specification
Immunogen: Recombinant GST-fused rat Nup153 (610-1191aa)
Epitope: 610-1191 aa (Zn finger and FG repeats domain)
Isotype: Rat IgG2aκ
Form: Purified monoclonal antibody (IgG) 1 mg/ml in PBS 50% glycerol filter-sterilized
Specificity: Specific to human mouse rat and monkey Nup153 proteins. Other species are not tested.
Storage: -20℃ (long period -70℃)

References:
1. UllmanK.S. et al. (1999) “The nucleoporin Nup153 plays a critical role in multiple types of nuclear export.” Molecular Biology of the Cell 10: 649-664.
2. LimR.Y. et al. (2007). Nanomechanical basis of selective gating by the nuclear pore complex. Science 318: 640-643.