anti-Psm1(S. pombe)antibody、rabbit antibody

Code:63-137

Schizosaccharomyces pombe Psm1 is a component of protein complex called cohesin which is required for sister chromatid cohesion during cell cycle and in DNA repair. The cohesion complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase the complex is cleaved and dissociates from chromatin allowing sister chromatids to segregate. S. pombe cohesin complexes are composed of the Psm1 and Psm3 heterodimer attached via their hinge domain Rad21 which link them and Psc3 which interacts with Rad21. Cohesin subunits are enriched in broad centromere region.

anti-Rad21(S. pombe)antibody、rabbit antibody

code:63-139
Rad21 protein (628 aa 67.8 kDa) is a cleavable component of the cohesin complex involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition this protein is cleaved by Cut1 and dissociates from
chromatin allowing sister chromatids to segregate. Also involved in the DNA double-strand-break (DSB) repair system Hyperphosphorylated during S and G2 phases.
Proteolytic cleavage of a fraction of hyperphosphorylated form at the onset of anaphase may be essential for the proper progression of anaphase and sister chromatid separation. Belongs to the rad21 family conserved to mammals.
Applications
1. Western blotting (1/1000~1/4000 dilution) (Ref.1 & 2)
Not tested for other applications
Immunogen: Recombinant full-size S. pombe Rad21 protein (His-tagged) expressed in E. coli
Specificity: Reacts with S. pombe Rad21 protein. Not tested with other species
Form: Rabbit antiserum added with 0.05 % sodium azide
Storage: Shipped at 4℃ and stored at -20℃ or below

anti-Sds22(S. pombe)antibody、rabbit antibody

Code:63-141

Schizosaccharomyces pombe Sds22 protein contains leucine-rich repeats and physically interacts with the catalytic subunits of two type 1 protein phosphatases (Dis2 and Sds21). Sds22 is a regulatory subunit of these phosphatases and the Sds22-bound phosphatases carry a key phosphatase activity essential for the progression from metaphase to anaphase. Sds22 is essential for cell viability and in its absence cells were blocked in metaphase. Sds22 protein is predicted to form a repeating helical rod that is capable of enhancing a PP1-dependent dephosphorylation activity.

anti-Sds23(S. pombe)antibody、rabbit antibody

Code:63-143

Schizosaccharomyces pombe Sds23 protein functions to facilitate the progression in anaphase in mitotic cell cycle. For initiating anaphase in fission yeast PP1 (protein phosphatase 1) and 20S cyclosome/APC are required. Thesds23 gene is a multicopy suppressor for mutations in PP1 and the 20S cyclosome/APC implying that the gene dosage increase can relieve the requirement for PP1 and the cyclosome/APC for the onset of anaphase. The sds23 gene encodes a 408 aa product and appears to be conserved. The sds23 gene is not essential for cell viability but in the sds23 deletion mutant the progression of anaphase and cytokinesis are retarded and cell shape is aberrant. Therefore Sds23 protein is involved in progression in anaphase as well as in cytokinesis and cell shape control. Sds23 is neither physically bound to PP1 nor a subunit of the cyclosome. It possibly regulates the PP1 and 20S cyclosome/APC in an unknown fashion.

anti-Mis13 (human) antibody rabbit polyclonal

70-101

Storage: Ship at 4℃ and store at -20℃.

Validation of Reactivity: Specificity has been validated with siRNA of human DSN1 for WB and IF. Reacts with mouse rat

Immunogen: Recombinant GST-DSN1 (1-356 aa)

Applications:
1) Western blotting (1000 fold dilution)
2) Immunofluorescence-staining (1000 fold dilution)

Product: Rabbit antiserum added with 0.05% sodium azide. Anti-GST antibodies have been removed from the serum with affinity column conjugated with GST

Background: DSN1 / Mis13 (human: 356 aa. 40 kDa) is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. Defects in kinetochore proteins often lead to aneuploidy and cancer. It is a component of the MIS12 complex composed of MIS12 DSN1 NSL1 and PMF1 and also interacts with CASC5 CBX3 and CBX5. Note that DSN1 protein is expressed in actively dividing cells.

anti-Rpn3(S.cerevisiae)antibody rabbit serum

code:62-201
Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1).Rpn3p is essential non-ATPase regulatory subunit of the 26S proteasome lid similar to the p58 subunit of the human 26S proteasome ( 2 3 4).
Applications
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation
3) Not tested for other application
Specification
Product: Rabbit antiserum
Immunogen: Recombinant yeast Rpn3p expressed in E. coli
Form: Rabbit serum added with 0.05 % sodium azide
React with: S. cerevisiae Rpn3p. Not tested with other species
Storage: -20℃. To avoid repeated freezing and thawing store the antibody in aliquots.
Reference: This product was used in ref. 3 and 4
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Glickman MH et al. A subcomplex of the proteasome regulatory particle required for ubiquitin-conjugate degradation and related to the COP9-signalosome and eIF3. Cell 94 :615-23( (1998)
4. Isono E et al. Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae. J Biol Chem. 279:27168-76 (2004)

anti-Rpn5(S.cerevisiae)antibody affinity purified

code:62-203
Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Rpn5p is essential non-ATPase regulatory subunit of the 26S proteasome lid similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit Rpn7 ( 2 3). It consists of 445 amino acids with molecular weight of 51768.
Applications
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation 3) Not tested for other applications
Specification
Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn5p
Immunogen: Recombinant yeast Rpn5p expressed in E. coli
Form: Purified IgG in 100 mM NaCl 10 mM Tris-HCl pH 7.4 0.05 % sodium azide
Reactivity: S. cerevisiae Rpn5p. Not tested with other species
Storage: -20℃. To avoid repeated freezing and thawing store the antibody in aliquots.
Reference: This product was used in ref. 3
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome. Mol. Biol. Cell 18: 569 (2007)

anti-Rpn7(S.cerevisiae)antibody affinity purified

code:62-205
Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa and is highly conserved among eukaryotic organisms. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Rpn7 is one of the lid subunits of the 26 S proteasome regulatory particle (1 2). The RPN7 gene is known to be essential and required for the integrity of the 26 S complex by establishing a correct lid structure (3 4).
Applications
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation 3) Not tested for other applications
Specification
Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn7p
Immunogen: Recombinant yeast Rpn7p expressed in E. coli (Ref. 4)
Form: Purified IgG in 100 mM NaCl 10 mM Tris-HCl pH 7.4 0.05 % sodium azide
Reaction: S. cerevisiae Rpn7p. Not tested with other species
Storage: -20 C. To avoid repeated freezing and thawing store the antibody solution in aliquots.
Reference: This product was used in ref. 3 and 4
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E et al. Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae J Biol Chem. 279:27168-76 (2004)
4. Isono E et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasomeMol. Biol. Cell 18: 569 (2007)

anti-Nob1(S.cerevisiae)antibody affinity purified

Code: 62-211

Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Nob1p is essential nuclear protein required for biogenesis of the 26S proteasome (2). Nob1p is speculated to serve as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and to be degraded upon the maturation of the 26S proteasome (3). Nob1p is composed of 459 amino acid residues.

anti-Nas6/p28 (S.cerevisiae) antibody affinity purified

Code:62-213

Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. NAS6 (p28) is a proteasome-interacting protein which acts as a chaperon to assemble the base sub-complex of the 19S RP (2). NAS6 is composed of 226 amino acid residues with calculated molecular masses of 24.4kD (3).
Applications
1) Western blotting (x 5000 fold dilution)(Fig.1) 2) Immunoprecipitation
3) Not tested for other applications
Product: Rabbit polyclonal antibody affinity purified with recombinant Nas6
Immunogen: Recombinant yeast Nas6 expressed in E. coli
Form: Purified IgG in PBS 1 mg/ml BSA 0.09 % sodium azide 50% glycerol
Reactivity: S. cerevisiae Nas6 not tested with other species
Storage: -20℃