anti-KDM5A/RBP2/JARID1A antibody mouse monoclonal (9A6)

Code:71-175

RBP2 (1690 aa 192 kDa) was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji AT rich interactive domain 1A) and KDM5A (gene name). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase.

anti-RBP2/JARID1A antibody mouse monoclonal (18E8)

Code:71-177

RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase (1 2 & 3).

Anti-Dnmt1 (1037-1386) antibrabbit polyclonal) ody affinity-purified (

Code:70-203

Dnmt1 (DNA (cytosine-5-)-methyltransferase 1) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Hypermethylation in promoter regions is asssociated with repression of gene expression. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1 2). This antibody was raised by Prof. S. Tajima of Osaka Univ. (3 4)
Applications
1) Western blotting (0.2～1 ug /ml)
2) Immunoprecipitation. Especially good for denatured protein and useful for ChiP assays
3) Indirect immuno-fluorescence staining
Properties of the product

Antigen: Recombinant mouse Dnmt1 (amino acids 1037-1386) purified by SDS-PAGE .

Antibody: Affinity-purified with the recombinant Dnmt1(1037-1386)

Reactivity: mouse human xenopus

Form: 1 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)

Storage: -20℃ (long period; -80℃)

Anti-EID1 antibody monoclonal (#2)

Code:71-190

EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation.

Anti-Dnmt3b antibody rabbit polyclonal affinity-pure

code:70-206

Dnmt3b (DNA (cytosine-5-)-methyltransferase 3b) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics).　Dnmt3b is thought　to function in de novo methylation rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene　cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
This antibody was prepared and characterized by Prof. S. Tajima of Osaka Univ. and used in Ref.2~4.
Applications
1) Western blotting (1000-fold dilution)
2) Immunoprecipitation (1000-fold dilution) useful for ChiP assays
3) Indirect immuno-fluorescence staining (1000-fold dilution)
4) Immunohistochemistry (1000-fold)
Properties of the product
Immunogen: Highly purified recombinant mouse Dnmt3b (amino acids 1-181) (accession no. AF068626）
Antibody: Affinity-purified with the recombinant Dnmt3b
Reactivity: Mouse rat and human
Form: 1 mg/ml in PBS 50% glycerol 0.05% sodium azide (and trace of ammonium sulfate)
Storage: -20℃ (long period; -80℃)

Human Epidermal Growth Factor (EGF) active

code:03-001、03-001-5

EGF is a polypeptide growth hormone which plays an important role in the regulation of growth proliferation and differentiation of a wide range of cells by binding to its receptor EGFR (epidermal growth factor receptor). Through its binding to cell surface receptors EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT RAS/ERK and JAK/STAT pathways.
The full-length human EGF was expressed in E.coli and highly purified by the propriety chromatographic procedures. This product has the same amino acid sequence with the mature human EGF (6.35 kDa) and has no tag attached.
Applications
1. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human EGF receptor transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin cornea etc.
5. Widely used in cosmetics such as whitening anti-crinkle anti-aging etc.
Specification
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96
AQueous Assay; Promega) with NHEK cells (normal human epidermal keratinocytes) was
< 0.1 ng/ml corresponding to a specific activity of < 1 x 107 units/mg.
Purity: >95% as determined by SDS-PAGE (CBB staining)
Form: 0.74 mg/ml in PBS (10mM Na-phosphate 150mM NaCl) pH7.2 50% glycerol filter-sterilyzed
Storage: -20℃ (for long-term storage -70℃)

Human Fibroblast Growth Factor 1 (FGF-1 / acidic FGF) active

code:03-003、03-003-5

FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family which binds heparin. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development cell growth morphogenesis tissue repair tumor growth and invasion (ref.).

Applications
1. Use as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human FGF1 receptor transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Widely used in cosmetics such as whitening anti-crinkle anti-aging etc.
Product: Full length recombinant mature FGF-1 (15.8 kDa 140 amino acids) expressed in E. coli.
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96
Promega) with Balb/c3T3 cells was < 10 pg/ml corresponding to a specific activity of < 1 x 108 units/mg.
Purity: >98% as determined by SDS-PAGE (CBB staining)
Form: 2.0 mg/ml in PBS (10mM Na-phosphate 150mM NaCl) pH7.2 50% glycerol filter-sterilyzed
Storage: -20℃ (for long-term storage -70℃)

Keratinocyte Growth Factor ( KGF/ FGF7 ) human active

Code:03-005、03-005-5

Keratinocyte Growth Factor (KGF) also known as Fibroblast Growth Factor 7 (FGF-7) is a member of fibroblast growth factor (FGF) family. Although FGF-7 has heparin binding activity similar to FGF-1 its mitogenic activity is predominantly exhibited in keratinocytes. It is not effective to fibroblasts and endothelial cells.
The human FGF-7 lacking the signal sequence (1-31 aa) was expressed in E. coli and purified by the chromatographic procedures. This product is an intact enzyme without tag with 19 kDa size (Fig.)

Anti-Gtr2(S.cerevisiae) antibody rabbit serum WB

Code:62-351

Gtr2 (341 amino acids 38.6 kDa) is the yeast homologue of RagC identified in yeast and classified as a Ras-like small GTPase subfamily. In cytoplasm GTP-bound Gtr2 forms a heterodimer with Gtr1 which is involved in autophagy through mTOR signal pathway.

Applications
1) Western blotting ( 500~ 2000 folds dilution)
Not tested for other applications

Hep3B-miR122 cell line. For HCV virus propagation

Code:20-001

Key words: Propagation of human Hepatitis C Virus Cell culture-adapted HCV clone (HCVcc) miR122 Hepato cellular carcinoma line Hep3B.

Usage: Hep3B / miR122 cell line a permissive cell line for the robust propagation of HCVcc by the expression of miR122 in Hep3B cells.

Cell line stock: 5 x 105 cells /1 ml in CELLBANKER-1 (cryopresevation media from Wako-Chemical Osaka). Sent with dry-ice and store at -80℃

Growth medium: Dulbeccos modified Eagles medium (DMEM) supplemented with 100 U/ml penicillin 100 μg/ml streptomycin and 10% fetal bovine serum (FBS).