Anti-Cdk1 / Cdc2 (Xenopus) antibody rabbit polyclonal

Code:69-003

Storage： Shipped at 4°C and store at -20℃

Reactivity: Xenopus human

Applications
1) Western blotting (1/200-1/500 dilution)
2) Immunoprecipitation (1/100 dilution)

Immunogen: Synthetic peptide corresponding to C-terminal region of Xenopus Cdk1 protein (C-KSSLPDNQIRN) conjugated with KLH

Purity: Purified IgG

Form: 2.0 mg/ml in 1 x PBS and 50% glycerol

Background: Cdk1(cyclin-dependent kinase1) plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme while phosphorylation at Thr-161 activates it. Molecular mass is 34506

Data Link: UniProtKB P35567 (CDK1A_XENLA)

anti-PIAS4/PIASy antibody mouse monoclonal (10-24.6)

Code:70-510

Shipping and Storage : Shipped at 4℃ and store at -20℃.

Reactivity: Human PIAS4. Does not react with other PIAS.


Applications
1. Western blotting (1/ 2500)
2. Immunoprecipitation (1/50)
3. Immunofluorescent staining (1/250)

Immunogen: Recombinant His-tagged human PIAS4 (2-473)

Form: Purified IgG 0.5 mg/ml in PBS 10% glycerol 0.04% sodium
azide.

Isotype: Mouse IgG2a

Background: PIAS4 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase stabilizing the interaction between UBE2I and the substrate and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways including the STAT pathway the p53/TP53 pathway the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing.
Mediates sumoylation of CEBPA PARK7 HERC2 MYB TCF4 and RNF168. In Wnt signaling represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.Human PIAS4 consists of 510 amino acids with molecular mass of 56504 Da.

anti-LBR(Human、Mouse) antibody arrinity-pure

70-301

Backgroud:Lamins are nuclear membrane proteins that serve to maintain nuclear structure and functions.
Lamin B receptor (LBR) is localized in the nuclear envelope inner membrane and anchors the lamina and heterochromatin to the membrane (1). It may mediate interaction between chromatin and lamin B (2). The interaction with lamin and chromatin is regulated by phosphorylation.

Applications
1)Western blotting (0.2~1 ug /ml)
2)Immunoprecipitation.
3)Indirect immuno-fluorescence staining
Other applications were not tested

Properties of the product
Antigen: Highly purified recombinant mouse LBR (amino acids 1-81)
Antibody: Affinity-purified with the recombinant LBR
Reactivity: Mouse and human. Not tested with other species
Form: 1.0 mg/ml in PBS 50% glycerol.

Reference
1. Worman HJ et al (1988) A lamin B receptor in the nuclear envelope. Pro.Natl.Acad.Sci. USA 85:8531
2. Pyrpasopoulou A et al. (1996) The lamin B receptor (LBR) provides essential chromatin docking sites at the nuclear envelope. EMBO J. 15: 7108-19