Anti-Calmegin antibody rabbit polyclonal

code:73-034

Function: Calmegin plays an important role in sperm fertility. Binds calcium ions. Specifically expressed during male meiotic germ cell development.

Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100dilution).
Not tested for other applications.

Anti-IGSF8 antibody rabbit polyclonal

73-038

Product: Rabbit anti-IGSF8 antiserum added with 0.09% sodium azide

Reactivity: mouse

Applications:
1. Western blotting (1/500~1/1000 dilution))
2. Immunofluorescence staining (1/100 dilution).
3. Immunohistochemical Staining (1/100 dilution)


Function: IGSF8 may play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81 associates with CD82 to suppress prostate cancer cell migration regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
Molecular mass: 65011 Da with 611 amino acids

Anti-PDILT antibody rabbit polyclonal

code:73-051

Key words: PDILT testis-specific protein disulfide isomerase homolog CALR3 ADAM3 Sperm Fetilization Protein folding Endoplasmic reticulum
Function: PDILT cooperates with the testis-specific calreticulin-like chaperone calsperin (CALR3) in the endoplasmic reticulum and plays an indispensable role in the disulfide-bond formation and folding of ADAM3. Pdilt(-/-) mice were male infertile because ADAM3 could not be folded properly and transported to the sperm surface without the PDILT/CALR3 complex.
Molecular mass:67759 Da with 588 amino acids. N-glucosylated
Applications:
1. Western blotting (1/1000 dilution)
2. Immunoprecipitation (1/100)
3. Immunohistochemistry、Frozen section (1/100-1/500)
Not tested for other applications.

Anti-PMIS2 antibody rabbit polyclonal

code:73-055

Storage: Shipped at 4℃ and stored at -20℃
Key words: Pmis2 Sperm Fetilization
Function: PMIS2 might function both as the ultimate factor regulating
sperm transport into the oviducts and in modulating sperm–zona
binding.
Molecular mass: 11040 Da with 96 amino acids. Likely to be
N-glycosylated

Applications:Western blotting (1/1000 dilution)

Reactivity: mouse

Anti-SPACA1 antibody rabbit polyclonal

code: 73-062

Key words: Sperm acrosome membrane-associated protein 1 Spermiogenesis Acrosome globozoospermia Transmembrane Glycoprotein Phosphoprotein

Function: SPACA1 is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. Disruption of Spaca1 led to the disappearance of the nuclear plate a dense lining of the nuclear envelope facing the inner acrosomal membrane. This coincided with the failure of acrosomal expansion during spermiogenesis and resulted in the degeneration and disappearance of the acrosome in mature spermatozoa. SPACA1-deficient male mice are infertile.
Molecular mass: 33343 Da with 611 amino acids
Applications:
1. Western blotting (1/1000 dilution))
2. Immunofluorescence staining (1/100~1/500 dilution).
Not tested for other applications.

Anti-SPESP1 antibody rabbit polyclonal

73-065

Function: The disruption of Spesp1 was shown to cause an aberrant
distribution of various sperm proteins. SPESP1 is necessary to produce the
fully ‘fusion competent’ sperm.

Applications:
1. Western blotting (1/1000 dilution)
2. Immunofluorescence staining (1/100~1/500)

Reactivity: mouse

Anti-ADAM1B antibody rat monoclonal (#57)

73-010
Shipping and storage: Ship at 4℃ and store at -20℃. Do not freeze.

Function: May play a role in spermatogenesis sperm maturation and fertilization..

Molecular mass: 89369 with 806 amino acids. N-terminal signal peptide with 33 amino acids from this protein is processed to give propeptide which may undergo further processing.

Reactivity: mouse

Applications:
1.Immunocytochemistry (1/100 – 1/300)
2. Immunohistochemistry (1/100~1/300)
Not suitable for western blotting..

Immunogen: Mouse sperm

Reactivity: Mouse. Not tested with other species.

anti- IZUMO1 antibody rabbit polyclonal

Code:73-042

Key words: Acrosome reaction Membrane fusion Protein trafficking IZUMO1 Sperm–egg fusion

Function: Essential sperm cell-surface protein required for
fertilization by acting as a ligand for FOLR4/JUNO receptor on egg.

The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane fusogen

Molecular mass: 44885 Da with 307 amino acids. Post-translational modification; Processing of N-terminal signal peptide with 21 amino acids. N-Glucosylation and phosphorylation.

Expression: Small amount in testis and abundant in sperm. No expression in other organs.
Applications:
1. Western blotting (1/1000)
2. Immunofluorescence staining (1/100~1/300)
3. Inhibition of sperm fusion with egg

Immunogen: KLH-conjugated synthetic peptides corresponding to the following three regions of human IZUMO 1.
[A]　C+KSLEKDYLPGHLDA
[B]　C+TQVPKEKATDSRQQ
[C]　C+ATTESSISLQPLQ

Anti- IZUMO1 antibody rat monoclonal (#125)

Code:73-045

Storage: Shipped at 4C and store at -20℃

Validation: Specificity validated with knock-out mice.

Reactivity: Mouse and human. Not tested in other species.

Immunogen: Mouse sperm
Isotype: Rat IgG2a kappa

Applications:
1. Western blotting (1/1000 dilution))
2. Immunoprecipitation (1/100 dilution)
3. Immunofluorescence staining (1/250 dilution))
Form: Purified IgG 1 mg/ml in PBS 50% glycerol. Filter-sterilized. Azide- and carrier- free.

Background: Essential sperm cell-surface protein required for fertilization by acting as a ligand for FOLR4/JUNO receptor on egg. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane fusogen

Molecular mass: 44885 Da with 307 amino acids (mouse). Post-translational modification; Processing of N-terminal signal peptide with 21 amino acids. N-Glycosylation and phosphorylation.

Expression; Testis (low level) and sperm (high level). Not expressed in other organs.

Anti-Rag C / RRAG C antibody rabbit polyclonal

code:71-023

Key Words: GTP-Binding Protein mTORC1 TOR signaling cascade

Function: Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids

Molecular mass: 44224 with 399 amino acids. Rag C forms a functional complex with Rag A and Rag B.

Cellular localization: Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus depending on the bound nucleotide state of associated RagA.

Post-translational modification: Acetylation and phosphorylation

Applications
1) Western blotting ( 1000~ 2000 folds dilution)
2) Immunofluorescence staining (1/100~1/500)
Not tested for other applications