anti-SARS spike glycoprotein antibody (clone 3A2)

Code:65-101

Background:A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by infecting mouse with SARS virus and as the clone which produces the antibody that specifically reacts with the virus-infected cell in the laboratory of Prof. K. Ikuta of Osaka University. Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.
Applications
1) Western blotting (0.1~0.3 μg/ml) 2) Immunofluoresece
staining (IHC) 3) ELISA
Isotype: IgG2b (kappa)
Form: Purified IgG 1 mg/ml in PBS (-) 50% glycerol filter-
sterilized azide free

References: This product has been used in references 2.
1. Satija N Lal SK The molecular Biology of SARS coronavirus. Ann. N.
Y. Acad. Sci. 1102 26-38 (2007)
2. Yamate M. et al. Establishing of Vero E6 cell clones persistently
infected with severe acute respiratory syndrome coronavirus.
Microbes and Infection 7:1530-1540 (2005)

HIV-1 p24 ELISA Kit “BioAcademia”

Product code 80-001 No Stock

This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwich ELISA (Enzyme Linked Immunosorbent Assay) method. p24 antigen is a structural protein of HIV-1 so the virus titer in the sample could be implicated from the measurement.

[Principle of the test]
This kit is based on the principle of a sandwich ELISA method that uses an antibody coated plate as a solid phase and a biotinyl antibody and a peroxidase-labeled streptavidin.

[Advantage of this kit]
○ p24 of subtype AE can be measured at the same sensitivity as subtype B because affinity purified polyclonal antibody raised against the full length recombinant p24 is used.
○ Risk-free kit because neither patient sera nor active virus products are used.
○ Assay can be performed at room temperature.

[Preservation and Expiration Date]
Storage : 2 ~ 8℃ (Please do not freeze.)
Expiration date for use : 1 year

[Required Reagent and Apparatus and Equipment]
1. Deionized water
2. Test tubes or microtubes (for sample
preparation)
3. Micropipettes and tips
4. Microplate reader

anti-HCV Core protein antibody mouse monoclonal (H6-29)

Code:65-052

HCV core protein is not only a component of nucleocapsid but also has multiple functions and is thought to be a pathogenic factor for hepatitis. It also participates in some cellular processes including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Applications
1. Western blotting 2. Immunohistochemistry
3. Immunofluorescence staining 4. ELISA

Immunogen: A part of the core region (nucleotides 369704amino acids
13-124) of HCV genotype 1b expressed in E. coli

Isotype: Mouse IgG2a kappa

Form: Purified monoclonal antibody(IgG1mg/mlin PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV core antigen of genotype 1b. Not tested in other genotypes

anti-HCV NS4a protein antibody mouse monoclonal (S4-13)

code:65-057

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3) . The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. NS3 serine proteinase is responsible for proteolytic processing of other non-structural proteins. NS4a protein (54 amino acids) forms a comlex with NS3 and functions as a cofactor for NS3 protease activity.

Applications
1. Western blotting
2. Immunofluorescence staining
3. ELISA
Other applications have not been tested.

Immunogen: A region of NS4 protein of HCV genotype 1b expressed in E.coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).

Isotype: Mouse IgG2b kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS4a protein of genotype 1b. Not tested with other genotypes.

anti-HCV NS5b protein antibody mouse monoclonal (NS5B-6)

65-067

Backgroud: Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. The polyprotein encoded is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining
3. ELISA Other applications have not been tested.

Immunogen: A region of NS5b protein of HCV genotype 1b expressed in E .coli

Isotype: Mouse IgG2b kappa

Form: 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

Anti-Hepatitis C virus (HCV) NS5a protein antibody monoclonal (8926)

Code:65-062

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining
Other applications have not been tested.

Immunogen: A region of NS5a protein of HCV genotype 1bexpressed in E. coli

Isotype: Mouse IgG 2a kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.

anti-HCV core protein antibody mouse monoclonal (H6-29) biotinylated

Code:65-053

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 94 kb which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. HCV core protein is not only a component of nucleocapsid but also has multiple functions and is a pathogenic factor for hepatitis. It also participates in some cellular processes including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Applications
1. Western blotting 2. Immunohistochemistry 3. Immuno-fluorescence staining
4. ELISA 5. FACS

Immunogen: A part of the core region (nucleotides 369-704 amino acids 13-124) of HCV genotype 1b expressed in E. coli

Conjugate: Bitin conjugated [biotin] / [IgG] = 6.6

Isotype: Mouse IgG2a kappa

Form: 0.7 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV core antigen of genotype 1b. Not tested in other genotype

anti-HCV core protein antibody mouse monoclonal (H6-29) FITC-conjugated

Code:65-054

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9.4 kb which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. HCV core protein (191 aa) is not only a component of nucleocapsid but also has multiple functions and is thought to be a pathogenic factor for hepatitis. It also participates in some cellular processes including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Applications
1. Western blotting 2. Immunohistochemistry 3. Immunofluorescence staining
4. ELISA 5. FACS

Immunogen: A part of the core region (nucleotides 369-704 amino acids 13-124) of HCV genotype 1b expressed in E. coli Conjugate: FITC conjugated [FITC] / [IgG] = 6.7

Isotype: Mouse IgG2a kappa

Form: 1.6 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV core antigen of genotype 1b. Not tested in other genotypes

anti-HCV NS4a protein antibody monoclonal (S4-13) biotin conjugated

Code:65-058

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. The polyprotein encoded is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. NS4a protein (54 amino acids) forms a comlex with NS3 and functions as a cofactor for NS3 protease activity.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS4 protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).

Conjugate: Biotin conjugated [biotin] / [IgG] = 11.3

Isotype: Mouse IgG2b kappa

Form: 0.6 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS4a protein of genotype 1b. Not tested in other genotypes.

anti- HCV NS4a protein antibody mouse monoclonal (S4-13) FITC conjugated

Code:65-059

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. The polyprotein encoded is processed into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. NS4a protein (54 amino acids) forms a comlex with NS3 and functions as a cofactor for NS3 protease activity.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS4 protein of HCV genotype 1b expressed in E.coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).
Congugate: FITC conjugated [FITC] / [IgG] = 10.4

Isotype: Mouse IgG2b kappa

Form: 1.4 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS4a protein of genotype 1b. Not tested in other genotypes..