anti-HIV-1 Gag p17 antibody. guinea pig serum

Code:65-010

Product: Polyclonal guinea pig antiserum raised against full-size recombinant p17 of HIV-1 (no tag)
Applications: Western blotting (X1000-3000). Immunoprecipitation. ELISA. IHC
Reactivity: Reacts with p17 as well as the Gag precursors such as p55
Form: Undiluted serum added with0.09% sodium azide.
Storage: Shipped at 4℃ or -20℃ and store at -20℃

HIV-1 protease functional

Code:05-013

Shipping and Storage: Shipped with dry-ice and stored at -80℃.

When necessary freezing (with liquid or ethanol in dry-ice) and thawing (in water at room temperature with shaking) should be done rapidly.

Product: Full-size functional recombinant HIV-1 protease purified from E. coli

Applications
1.Functional studies such as screening for inhibitors of HIV-
protease for AIDS drug discovery
2.Antigen for anti-HIV 1 antibody for Western blotting and
ELISA
3.Control as HIV 1 protease for SDS=PAGE
Other applications have not been tested.

Stock solution: 0.2 mg /ml in 20 mM Tris 20 mM MES 0.2 M NaCl 1 mMEDTA 1 mM DTT 10% Glycerol pH 6.5

Reaction buffer: 20 mM Tris-HCl (pH 6.8) 1 mM EDTA 1 mM DTT 0.1% Triton X-100 10% Glycerol

Background: HIV-1 protease is the aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell. Also cleaves Nef and Vif probably concomitantly with viral structural proteins on maturation of virus particles. Hydrolyzes host EIF4GI and PABP1 in order to shut off the capped cellular mRNA translation. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and to evade host immune response.