Cholera toxin A subunit from Vibrio Cholerae active

01-521

Backgroud:The main enterotoxin known as cholera toxin interacts with G proteins and increases cyclic AMP in the intestinal lining to open ion channels . Cholerae toxin is a complex consisting of one molecule of A subunit (27.2 kD) and 5 molecules of B subunit (11.6 kD).

Applications
1. ADP ribosylation assay
2. Study of signal transduction

Purity: No contamination of the B-subunit as examined by biological and SDS-PAGE
Form：1.6 mg/ml in 20 mM Tris-HCl pH 8.2、0.2M NaCl 10% glycerol filter-sterilized
Storage: -80℃

anti-c-Myc p-Ser62(human)antibody、monoclonal(33A12E10)

code:71-161
Storage temperature: Shipped at 4℃ or -20℃ and stored at -20℃.
Avoid freezing by storing below -25℃

Validation and caution: Specificity of this antibody have been independently validated and caution of the use described in Ref.3
which is recommended to be read by the users..
Reactivity: Human and mouse.
Immunogen: Synthetic peptide containing phospho-Ser62 of cMyc

Applications
1. Western blotting (~1ug/ml Fig.1)
2. Immunofluorescence staining (0.5~1 μg/ml Fig.2)
3. Immunohistochemistry (5 μg/ml Perform heat mediated antigen
retrieval with citrate buffer pH 6 before formalin treated
paraffin embedded sectioning)
4. Flow cytometry (Use 1 μg for 106 cells)
5. Indirect ELISA (Assay dependent concentration)　　

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS 50% glycerol. Azide- and carrier-free.
Isotype: Mouse IgG2b (κ)

anti-Rpn5(S.cerevisiae)antibody affinity purified

code:62-203
Background: The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation DNA repair signal transduction apoptosis and metabolic regulation (1). Rpn5p is essential non-ATPase regulatory subunit of the 26S proteasome lid similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit Rpn7 ( 2 3). It consists of 445 amino acids with molecular weight of 51768.
Applications
1) Western blotting (~1000 fold dilution) 2) Immunoprecipitation 3) Not tested for other applications
Specification
Product: Rabbit polyclonal antibody affinity purified with recombinant Rpn5p
Immunogen: Recombinant yeast Rpn5p expressed in E. coli
Form: Purified IgG in 100 mM NaCl 10 mM Tris-HCl pH 7.4 0.05 % sodium azide
Reactivity: S. cerevisiae Rpn5p. Not tested with other species
Storage: -20℃. To avoid repeated freezing and thawing store the antibody in aliquots.
Reference: This product was used in ref. 3
1. Hershko A. and Ciechanover A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67 425-479 (1998)
2. Finley D et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome. Mol. Biol. Cell 18: 569 (2007)

Streptolysin O (Hemolytic streptococcus) active Useful for introduction of molecules into living cells

01-531

Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. It forms a big hole on the cell membrane which enables the introduction of protein inside living cells in vivo .

Storage: Ship at 4℃ or -20℃ and store at -20°C
(-80°C for long-term storage).

Applications:
1) Antigen for the measurement of anti-streptolysin O antibody (ASO) (diagnostic reagent)
2) Reagent for membrane pore formation to introduce small-to-
macromolecules into living cells

Form: 1 mg/ml in PBS (-) 1 mM DTT 50% glycerol sterilized by filtration

anti-Mrc1 (S. pombe) antibody rabbit antiserum

Code:63-151

Backgroud: Mrc1 (Mediator of replication checkpoint 1) is a S-phase checkpoint
protein of Schizosaccharomyces pombe found at replication forks required for
phosphorylation and activation of Cds1 during DNA replication stress where it forms
a replication-pausing complex and is phosphorylated by Rad3 and Tel1 kinases. The
phosphorylated form of Mrc1 is detected by a change in the electrophoretic mobility.
Human homolog of Mrc1 is claspin.
Applications
1) Western blotting (x3000 dilution)
2) Other applications were not tested.
Specification
Immunogen: Purified　recombinant protein containing amino acids 1～317 of Mrc1
Form; Undiluted rabbit antiserum added with 0.05 % sodium azide

Reference: This product is described and used in reference 1
1. Zhao H. Mol Cell Biol 23: 8395 (2003)

Anti-Dnmt1(1-248)antibody affinity-purified

Code:70-201

Shipping and Storage temperatures: Ship at 4℃ or -20℃ and store at -20℃. Do not freeze

Applications
1)Western blotting (0.2~1 ug /ml)
2)Immunoprecipitation. Especially good for native form of Dnmt1
and useful for analysis of the protein complex containing Dnmt1.
One ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1.
3)Indirect immuno-fluorescence staining. Excellent. Low background
image will be obtained by using the antibody at 5000 fold
dilution.
4) Chromatin Immuno-Precipitation (Assay dependent)

Antigen: Highly purified recombinant mouse Dnmt1 (amino acids 1-248) soluble form

Antibody: Affinity-purified with the recombinant Dnmt1

Reactivity: Mouse and human. Not tested for other species (1-248).

Form: 1 mg/ml in PBS 50% glycerol

Data Link: UniProtKB/Swiss-Prot P13864 (DNMT1_MOUSE)

Anti-HB-EGF(human) antibody monoclonal(4G10)

Code:71-501

Storage: Shipped at 4℃ and store at -20℃　（Do not store below -20℃）

Product: Mouse monoclonal antibody produced in serum-free medium and purified by combination of chromatography

Reactivity: React with human but not with mouse
Immunogen: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Epitope: Amino acids 136-149 in the EGF domain

Applications The antibody can react with both soluble and cell-surface attached forms of HG-EGF.
1) Western blotting (0.2~1 ug /ml) non-denaturing condition.
2) Immunoprecipitation (2 ug/ml)
3) Immunofluorescence staining (5~10 ug/ml)
4) Immunohistochemistry (assay dependent)
5) Inhibition of HB-EGF ectoderm shedding (Ref 1)
6) Inhibition of Diphtheria Toxin binding to HB-EGFR (Ref 1)


Isotype: IgG1 (mouse)
Form: 1 mg/ml in PBS 50% glycerol filter-sterilized azide-free

Anti-HB-EGF(human) antibody monoclonal(4G10) biotinylated

Code:71-503

Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor HB-EGF (1 2). The former functions as juxtacrine and the latter as paracrine growth factor. HB-EGF activates EGFR and ErbB4 and promotes the development of many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin (3). Non-toxic mutant of diphtheria toxin CRM197 inhibit HB-EGF function which is elevated in most ovarian cancer is being tested as an anticancer drug (4).

Applications
1)Western blotting (0.2~1 ug /ml) 2) Immunoprecipitation.
3)Indirect immuno-fluorescence staining
Properties of the product
Antigen: Recombinant human HB-EGF ectodomain expressed in SF21 cell.
Antibody: Produced in serum-free medium and purified by combination of chromatography
Isotype: IgG1(mouse) Epitope: EGF domain Reactivity: React with human but not with mouse
Form: Biotinylated IgG (biotin/IgG = 7.5) 0.6 mg/ml in PBS 50% glycerol filter-sterilized azide-free

Reference:
1. Higashiyama S. et al. Science 251: 936 (1991)
2. Prenzel N. et al. Nature 402: 884 (1999)
3. Iwamoto R. et al. EMBO J 13: 2322 (1994)
4. Miyamoto S. et al. Cancer Res. 64:5720 (2004)

Related product: # 71-501 Anti-HB-EGF (human) antibody monoclonal (4G10). # 01-515 Diphtheria toxin mutant CRM197. # 01-517 Diphtheria toxin..

Anti-EID1 antibody monoclonal (#26)

Code:71-185

EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein which interacts
with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and
CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the
transcription of genes required for cellular differentiation.

Anti-Shugoshin 1 (S. pombe) antibody

Code：63-154

Shugoshin 1 a meiosis-specific protein protects centromeric Rec8 from degradation during first meiotic division (meiosis I) in fission yeast Schizosaccharomyces pombe.
Applications: 1) Western blotting (1000 fold dilution)
2) Immunofluoresece staining （100 fold dilution）
Antibody: Recombinant Shugosin 1 was used as immunogen for rabbit and affinity purified with the recombinant Shugosin1.
Form: 0.2 mg/ml Affinity purified anti-Shugosin 1 antibody in 1 mg/ml BSA PBS (pH 7.2) 50% glycerol filter-sterilized azide free.