Adenylate cyclase from Bordetella pertussis active

01-501

Storage: Shipped with ice-pack or dry-ice and store at -20℃ or
-80℃ (for longer period).

Applications:
1)Functional studies on the cAMP- signaling pathway (10~100 ng/ml)
2)Antigen for western blotting and ELISA
3)SDS-PAGE marker as native adenylate cyclase

Form: 550 μｇ/ml in 50 mM Tris-HCl(pH8.0) 6M urea 150 mM NaCl

Purity: More than 90% purity by SDS-PAGE (CBB staining)

Dermonecrotizing toxin from Bordetella bronochiseptica active

01-505

Product:This Dermonecrotic toxin (DNT) is derived from Bordetella
bronchiseptica. This toxin activates smallGTP-binding proteins such as
RhoRac and Cdc42 through deamidation or polyamination for downstream
signaling pathways.

Applications:1)For the studies of the signaling pathways mediated
small GTP-binding proteins.2) Antigen for the detection of
Bordetera infection.

Activity: DNT at 5 ng/ml induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.

Form: 0.53 mｇ/ml in 50 mM Tris-HCl (pH 8.0) 6 M urea 150 mM NaCl .

Pasteurella multocida toxin active

01-507

Product: PMT( or dermonecrotic toxin (146 kD)) activates a subunit of the Gq and G12/13 protein which then activates the phospholipase C-b1 that controls several specific downstream signaling events.

Applications:For the studies on the GTP trimer bound protein dependent signaling pathways.

Form: 370 ug/ml in 50% glycerol 5mM Tris-HCl(pH7.5) 0.1M NaCl

Storage: -20℃ (long period; -80℃)

Clostridium perfringens toxin active

01-509

Clostridium perfringens enterotoxin (CPE) is a protein toxin produced by Gram-positive bacteriaC. perfringens. CPE destroys cell membrane structure of animals by its phospholipase activity after binding to the membrane of Claudin family proteins which are components of tight junction of epithelial cell membrane. CPE binds to Claudins 3 4 6 7 8 and 14 but not to Claudins 1 2 5 and 10.
　
Storage: Ship at 4℃ or -20℃ and store at -20℃ or
-80℃ (for long-term storage).

Applications
1. For the study of Claudin localization in cells
2. For the study of tight junctions
3. Western blot
4. SDS-PAGE .
5. ELISA

Form: 280 µg/ml in 50% glycerol 10 mM sodium phosphate buffer (pH 6.7)

Cholera toxin from Vibrio Cholerae active

01-511

Product: Highly purified (more tha 90% purity) from cultured medium
of Vibrio cholera strain 569B
The toxin is composed of one molecule of A-subunit (27.2
kD)responsible for binding to ganglioside of target cell and five
molecules of B-subunit (11.6 kD) constitutively activating adenylate
cyclase

Application:
1. Detection of GTP-binding protein Gsα
2. Detection of a low molecular weight GTP-binding protein ARF
3. To increase intracellular concentration of cyclic AMP of cultured
cell
Form: 1.0 mg/ml in 50 mM Tris-HCl (pH7.5) 0.2 M NaCl 1mM Na2EDTA
10% Glycerol　

Botulinum C3 enzyme from Clostridium botulinum active

01-513

Product: Highly purified (more than 90% pure) from growth medium of
Clostridium botulinum C strain
1.33 mg/ml in 50% glycerol 5 mM sodium phosphate buffer (pH 6.0)

Property: The C3 enzyme ADP-ribosylates a small GTP-binding protein Rho
and shuts down the downstream signal transduction pathway

Application: For the study on the signal transduction pathway dependent on Rho protein

Storage: -20℃　（long period -80℃)

Diphtheria toxin mutant CRM197 active but nontoxic

01-515

Product:The CRM197 was highly purified (>95% pure) from cultured medium of Corynebacterium diphtheriae mutant CRM197.

Properties: CRM197 can bind to HB-EGF and inhibit the growth stimulating activity of HB-EGF without showing cellular toxicity.

Applications:　1. Inhibitor of HB-EGF
2. Candidate of anticancer drug for tumors whose growth
depends on HB-EGF

Form: 1.0 mg/ ml in 20 mM Tris-HCl (pH 7.2) 150 mM NaCl

Ship with dry-ice and store at -80℃

anti-Swi6 (S. pombe) antibody rabbit serum

Code:63-101

Product: Rabbit serum raised by immunizing full-size Swi6 protein of S. pombe expressed in E.coli

Application : For the studies of RNAi mechanism
1. Western blotting (x 2000~10000 dilution)
2. Immunoprecipitation

Specifications
State: Undiluted antiserum added with 0.09% sodium azide
Reactivity: Swi6 protein of S. pombe
References

1. Yao MC et al. Science 300:1581 (2003)
2.Ekwall K et al. Science 269:1429 (1995)
3. Wang G et al. Mol Cell Biol 20:6970 (2000)

anti-RanBPM/BP9 (human) antibody rabbit serum

71-001

Storage : Shipped at 4℃ and store at -20℃

Validation: Indpendently validated in Ref.3 for IF.

Reactivity: human monkey rodents dog

Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)
4. Immunohistochemistry (1/200 dilution. Perform heat mediated
antigen retrieval with citrate buffer (pH 6) before formalin
treated paraffin embedded sectioning)

Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex. The protein consists of 729 amino acids and is identified by Western blotting as an apparent molecular mass of 95 kD (see below). It is involved in nucleation of microtubules and controls cell growth by interacting with many protein factors.

anti-RanBP2(human)antibody　rabbit serum

Code:71-003
Storage : Shipped at 4℃ and store at -20℃
Reactivity: human monkey rodents dog
Immunogen: Recombinant human RanBP9 from Phe133 to Tyr 229.

Applications
1. Western blotting (1/1000- 1/ 2000 dilution)
2. Immunoprecipitation (assay dependent concentration)
3. Immunofluorescence stainig (1/200~1/1000 dilution)
4. Immunohistochemistry (1/200 dilution. Perform heat mediated antigen retrieval with citrate buffer (pH 6) before formalin treated paraffin embedded sectioning)
Form: Undiluted antiserum added with 0.09% sodium azide

Background: RanBPM (RanBP9) was identified as a protein that interacts with small GTP-binding protein Ran and forms a 670 kD multiprotein complex.