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Code:64-030
Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi Go and Gt and prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26 kDa) S2 (22 kDa) S3 (22 kDa) S5 (12 kDa) and two molecule of S4 (12 kDa). This product was highly purified (>90% pure) from Bordetella pertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below).
Applications:
1. Western blotting (1/2000~1/10000 dilution)
2. ELISA (1/10000~1/20000 dilution)
3. Dot blotting (1/2000~1/10000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralising (Assay dependent)
Other applications have not been tested.
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