anti-HCV NS5a protein antibody monoclonal (8926) biotinylated

Code:65-063

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b (ref.4) expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 9.6

Isotype: Mouse IgG 2a kappa

Form: 0.8 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.

anti- HCV NS5a protein antibody monoclonal (8926) FITC conjugated

Code:65-064

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9400 bases which encodes a single polyprotein of about 3010 amino acids (1 2 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core envelope1 and 2 and p7) and six non-structural proteins (NS2 3 4a 4b 5a and 5b) necessary for viral replication. The primary function of NS5a is not known but from the comparative studies with other viruses it is predicted to play a role in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. FACS

Immunogen: A region of NS5a protein of HCV genotype 1b expressed in E. coli

Conjugate: FITC conjugated [FITC] / [IgG] = 4.8

Isotype: Mouse IgG 2a kappa

Form: Purified monoclonal antibody (IgG) 1.4 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5a protein of genotype 1b. Not tested in other genotypes.

anti- HCV NS5b protein antibody mouse monoclonal (NS5B-6) biotinylated

Code:65-068

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of hepatitis. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS5b protein of HCV genotype 1b expressed in E. coli

Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

Isotype: Mouse IgG2b kappa

Form: 0.7 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

anti- HCV NS5b protein antibody mouse monoclonal (NS5B-6) FITC conjugated

Code:65-069

Hepatitis C virus (HCV) is a small (55-65 nm in size) enveloped positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A non-B hepatitis. The polyprotein encoded is processed by host cell and viral proteases. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Applications
1. Western blotting 2. Immunofluorescence staining 3. ELISA 4. FACS

Immunogen: A region of NS5b protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli

Conjugate: FITC conjugated [FITC] / [IgG] = 6.0

Isotype: Mouse IgG2b kappa

Form: 1.3 mg/ml in PBS 50% glycerol filter-sterilized

Specificity: Specific to human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

Anti-HIV-1 p24 antibody rabbit polyclonal biotinylated IgG

Code:65-021

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus subtype B (2) and was highly purified by several steps of chromatography (3 4).
Using this antiserum in Western blotting the bands of 24 kD 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

Applications
1. Western blotting 2. Immunoprecipitaiton 3. Immunofluorescence　 staining 4. ELISA 　　　　　　　　　Not tested in other application

Form: Biotin conjugated IgG (0.9 mg/ml) in PBS 50% glycerol filter-sterilized
Storage:　Sent at 4℃ or -20℃ and store at -20℃

anti-Influenza A Virus NP monoclonal antibody (C43) Pantropic

65-110

Storage temperature: Ship at 4℃ and store at -20℃.

Specificity: Reacts with NP of all influenza A viruses including
seasonal H2N2 H3N2 and avian H5N1 H5N2 and H1N1 (seasonal pandemic
and swine). No cross reactivity with influenza B viruses.

Immunogen: Human Influenza A Virus (H2N2) Okada strain

Applications
1) Western blotting (300~1000 fold dilution)
2) Immuno-precipitation (100 fold dilution)
3) Immunofluorescent staining (200 fold dilution)
4) Immunohistochemistry (200 fold dilution)
5) ELISA (assay dependent)

Isotype: mouse IgG2A

Purity: Produced in serum-free medium and purified by proprietary
chromatography procedure under mild conditions. 90~95% pure by
SDS-PAGE

Form: 1 mg/ml in PBS 50% glycerol filter sterilized. No additive
nor carrier protein added

Anti-HHV-6 gQ1 antibody mouse monoclonal (119)

Code:65-200

Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV-6A and HHV-6B is 90% and they are now classified as distinct species.
HHV-6A has been described as more neurovirulent and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis.
HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally HHV-6B reactivation is common in transplant recipients which can cause several clinical manifestations such as encephalitis bone marrow suppression and pneumonitis.
gQ1 encoded by the U100 gene of HHV6 is glycoprotein complexes with gH gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80-kDa form (gQ1-80K) and a 74-kDa form (gQ1-74K) - only gQ1-80K but not gQ1-74K forms the ligand complex with gQ2 gH and gL. It associates with lipid rafts.

Anti-Influenza A Virus Nucleoprotein antibody C43) HRP-conjugated

Code:65-111

Influenza virus is an RNA virus which causes influenza and belongs to the family Orthomyxoviridae. Influenza virus is classified into three different genera influenzavirus A B and C. They all have similar structures and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the virion is made of a viral envelope containing two major glycoproteins hemagglutinin (HA) and neuraminidase (NA). Influenzavirus A is further classified into subtypes based on the surface glycoproteins HA and NA. Currently there are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome which is packaged in the form of ribonucleoprotein complexes.
Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein which encapsidates the negative strand viral RNA and is essential for RNA transcription replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1 in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins and these interactions have been shown to be essential for the viral lifecycle.

Anti-Influenza B Virus M1 antibody mouse monoclonal（6A11)

Code:65-155

Background: Matrix protein 1 (M1) plays critical roles in virus replication from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1 a non-raft-associated protein into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion where it binds the RNP. During virus entry into cell the M2 ion channel acidifies the internal virion core inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus where viral transcription and replication can take place.
M1 of Influenza B virus consists of 248 amino acids with molecular mass of 27415.

Anti-Influenza B Virus HA antibody mouse monoclonal（1H12）)

Code:65-160

Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2 releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Post-translational modification
HAo is consists of 584 amino acids with molecular mass of 63275. In natural infection inactive HA is matured into HA1 and HA2. By sequence similarity it is indicated to be palmitoylated.