anti-Streptolysin O antibody rabbit serum

Code:64-001

Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. In the hemolytic streptococci infection the antibodies to various proteins secreted from the bacteria such as toxins increase. For the diagnostic confirmation of SLO production by the infection the measurement of the antibody ASLO (Anti-Streptolysin O) is most widely used.

Applications
1) Western blotting (x2000~10000 dilution)
2) Neutralization of hemolysis by Streptolysin O (SLO)
3) Immunoprecipitaion
4) ELISA

*Most of the anti-Streptolysin O polyclonal antibodies (ASLO) sold in the current market were obtained by immunizing with SLO purified from the culture supernatant of hemolytic Streptococci. But the SLO preparations were contaminated with other secreted proteins such as NADase. This product is highly specific to SLO because of the purity of immunogen

Immunogen: His6-tagged full-size recombinant SLO of S. dysgalactiae subsp. equismilis H46A (group C hemolytic Streptococci) expressed and highly purified from E. coli

Reactivity: Streptolysin O of Group ACand G origins

Form: Undiluted serum added with 0.09 % sodium azide.

Storage: Shipped at 4℃ or -20℃ and store at -20℃

Anti-NADase (hemolytic streptococcus) antibody rabbit serum

Code:64-005

NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although its function as a toxin is largely unknown it has been suggested to be related to pathogenicity of acute infection (1). NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross-react each other. Upon infection of hemolytic streptococci the antibody titer to the NADase increases similarly to anti-SLO (Strreptolysin O) antibody.
Application
1) Western blotting (x 2000~10000 dilution)
2) Immunoprecipitation
3) Neutralization of NADase activity
4) ELISA

Anti-Diphtheria Toxin antibody rabbit serum

Code:64-010

Diphtheria toxin (535 aa 58 kDa) is an exotoxin secreted as a proenzyme by Corynebacterium diphtheriae the pathogen bacterium that causes diphtheria. The toxin gene is encoded by a bacteriophage. The proemzyme undergoes proteolytic nicking and reduction of disulfide bond giving rise to subunit A (21 kDa) and B (37 kDa). Subunit B (C-terminal) recognizes cellular receptor HB-EGF receptor and subunit A (N-terminal) catalyzes the ADP-ribosylation of eukaryotic elongation factor-2 (eEF2) inactivating this protein.
Applications:
1. Western blotting (1/2000 dilution).
2. ELISA (1/2000~1/7000 dilution. Could detect 1 ng or less of the toxin)
3. Immunoprecipitation (1/200~ 1/1000 dilution depending on assay)
4. Dot blotting (1/1000 dilution. Could detect 1 ng or less of the toxin) 　
Immunogen: Purified nontoxic mutant Diphtheria toxin CRM 197 (Ref 1 Product #01-515)

Diphtheria toxin active

01-517

Product:Highly purified (>95% pure) from cultured medium of
Corynebacterium diphtheriae strain PW8

Properties: It is a 53 kD protein consisting of two subunits linked by disulfide bridges It catalyzes ADP-ribosylation of eukaryotic elongation factor 2 and inhibits protein synthesis.

Applications:1. Inhibition of protein synthesis of eukaryotes
2. Putative anti-cancer drug utilyzing strong immunotoxin activity

Form:2~ 5 mg/ml in 20 mM Tris-HCl (pH 7.2) 150 mM NaCl10% Glycerol