(cDNA library) Planaria ; Planaria

Code:02-709

This cDNA library (plasmid DNA) is constructed from Planaria-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express genes in mammalian cells as it contains SV40 promoter. It also contains Ori for the plasmid replication and f1 ori which is necessary for ssDNA synthesis in E. coli and bacteriophage T7 and T3 promoter for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression levelof mRNA of the particular gene.)

(cDNA library) S.pombe ; After + HUγMMS h-L972

code:02-707

This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA derived from Schizosaccharomyces pombe strain h-L972 after treatment with HU(hydroxyurea) -radiation and MMS(methylmethane sulfonate). It is constructed by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pLZ3 vector used in this library can replicate both in S. pombe and in E. coli and express cloned genes not only in S. pombe but also in mammalian cells as it contains SV40 promoter. It also contains f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoter for RNA synthesis (see Figure and Ref.2).
Application
1. PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)
2. Cloning the cDNA by functional complementation of the corresponding mutant strains.

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 7.7 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃

(cDNA library) Human contact inhibition state

code:02-727

This cDNA library (plasmid DNA) is constructed from poly(A)+ RNA of human TIG-1 cells (fetal lung fibroblasts) at contact inhibition state. It is constructed by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express rat genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468
Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones:50 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃

(cDNA library) Human ; Fibroblast Primary culture

code:02-725

This cDNA library (plasmid DNA) is constructed from human TIG-1 cell (fetal lung fibroblast primary culture)-derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pBA3 vector used in this library has pUC ori which enables replication in E. coli and Ampr as a selection marker.

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

Specification
Quantity: 500 ng (40 ng/ul 13ul) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
Quality: 1) Number of independent clones: 8.6 x 106
2) Average insert size : longer than 1 kb
Storage: -20℃

(cDNA library) Human ; 293T

Code:02-729

This cDNA library (plasmid DNA) is constructed from human embryonic kidney 293T cell -derived poly(A)+ RNA by the Linker-Primer method (Ref.1) by Professor Hiroshi Nojima of Research Institute for Microbial Diseases Osaka University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I and BamHI (Bgl II)-Sma I adaptor.
The pAP3neo vector used in this library can express human genes in mammalian cells as it contains SV40 promoter. It also contains Ori of pUC plasmid required for replication in E.coli f1 ori which is necessary for ssDNA synthesis and bacteriophage T7 and T3 promoters for RNA synthesis (see Figure). GenBank Accession No. AB003468

Application
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions and preparation of probes etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)

DNA Size Markers 1 kbp DNA Ladders (1-10 kbp) 50 times

Code:02-400

The BioAcademia 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 1 kbp to 10 kbp. The 4 kb band has increased intensity to provide internal orientation. The mass of DNA in each band is provided (assuming a 0.45 μg/ 5 μl load) for approximate quantification in comparably intense samples of similar size.

DNA Size Marker 100 bp Ladders (100-1500 bp) 50 times

Code:02-420

The BioAcademia 100 bp DNA Ladder mixture is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1.5 kbp as for sizing PCR products. The 500 bp band has increased intensity (100 ng/5 ul) to provide internal orientation. The mass of DNA in each band in other bands is provided (assuming a 50 ng/ 5 μl load) for approximate quantification in comparably intense samples of similar size. To avoid annealing between the DNA bands all the bands have blunt-ends.